Alginate-templated microcapsules carrying heparin via layer-by-layer self-assembly

Juan Li, Xinrong Liu, Baoqing Zhu, Yingya Shao, Dongxu Zhao, Xinlin Yang*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

6 引用 (Scopus)

摘要

Electrostatic layer-by-layer self-assembly is a simple and effective approach to carry macromolecules into microscale carriers. Calcium-cross-linked alginate hydrogel microspheres were fabricated by an emulsification technique and then used as negatively charged templates for polyelectrolyte multilayer coatings to entrap heparin. The polycation was poly (allylamine hydrochloride) (PAH), while the polyanion was heparin, an anticoagulant to prevent the clotting of blood. The microcapsule was formed via coating {PAH/heparin}3 on Ca2+-cross-linked alginate hydrogel. The retention of alginate in {PAH/heparin}3 microcapsule was confirmed by fluorescence inverted microscopy and confocal laser scanning microscopy. The assembly of polyelectrolyte layers on alginate microspheres was monitored by Zeta Potential Analyzer. The concentration of heparin was detected by HPLC with IR-detector and organic acid column. It was found that heparin could be entrapped in the microcapsules in high efficiency, providing a useful delivery system which would be found potential application in antagonizing thrombosis.

源语言英语
主期刊名2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
出版商IEEE Computer Society
1420-1423
页数4
ISBN(印刷版)9781424417483
DOI
出版状态已出版 - 2008
活动2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008 - Shanghai, 中国
期限: 16 5月 200818 5月 2008

出版系列

姓名2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008

会议

会议2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
国家/地区中国
Shanghai
时期16/05/0818/05/08

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