Actinotalea soli sp. nov., isolated from Kubuqi Desert soil

Jie Du, Weiwei Ping, Ya Chen, Huancheng Pang, Pengze Bai, Siqi Cui, Jianli Zhang*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

2 引用 (Scopus)

摘要

A Gram-stain positive, facultatively anaerobic, motile rod-shaped strain, BY-33T, was isolated from a soil sample obtained from the Kubuqi Desert, PR China. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain BY-33T was most closely related to the genus Actinotalea, including Actinotalea ferrariae CF5-4T (98.2 % similarity), ‘Actinotalea subter-ranea’ HO-Ch2T (98.0 %), Actinotalea solisilvae THG-T121T (97.6 %), ‘Actinotalea bogoriensis’ 69B4T (97.5 %), Actinotalea fermentans MT (97.3 %) and ‘Actinotalea carbonis’ T26T (97.0 %). The strain grew at 0‒37 °C (optimum, 28–30 °C) and pH 6.0–11.0 (optimum, pH 9.0–10.0) and with 0‒8.0 % (w/v) NaCl (optimum, 3.0%) on tryptic soy agar. It had catalase activity, but no oxidase activity. The polar lipids of strain BY-33T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphati-dylinositol mannosides. The major respiratory quinone of strain BY-33T was MK-10 (H4). Its major fatty acids were anteiso-C15: 0, anteiso-C15: 1 A and C16: 0 . The genomic DNA G+C content of strain BY-33T was 73.0 mol% based on total genome calculations. The average nucleotide identity scores between the genomic sequences of strain BY-33T and the other species of the genus Actino-talea were found to be low (ANIm <85.0 %, ANIb <77.0 % and OrthoANIu <78.0 %). Furthermore, the digital DNA–DNA hybridization and average amino acid identity values between strain BY-33T and the closely related species ranged from 20.5 to 21.0% and from 62.2 to 72.2 %, respectively. Based on the results of phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain BY-33T represents a novel species within the genus Actinotalea, for which the name Actinotalea soli sp. nov. is proposed. The type strain is BY-33T (=CGMCC 1.17460T=KCTC 49362T).

源语言英语
文章编号005660
期刊International Journal of Systematic and Evolutionary Microbiology
72
12
DOI
出版状态已出版 - 2022

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Du, J., Ping, W., Chen, Y., Pang, H., Bai, P., Cui, S., & Zhang, J. (2022). Actinotalea soli sp. nov., isolated from Kubuqi Desert soil. International Journal of Systematic and Evolutionary Microbiology, 72(12), 文章 005660. https://doi.org/10.1099/ijsem.0.005660