Zhang, J., Fu, R., Xie, L., Li, Q., Zhou, W., Wang, R., Ye, J., Wang, D., Xue, N., Lin, X., Lu, Y., & Huang, G. (2015). A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation. Lab on a Chip, 15(19), 3891-3896. https://doi.org/10.1039/c5lc00488h
Zhang, Junqi ; Fu, Rongxin ; Xie, Liping 等. / A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation. 在: Lab on a Chip. 2015 ; 卷 15, 号码 19. 页码 3891-3896.
@article{f94a139eebd3488d8f977dc88e7e500c,
title = "A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation",
abstract = "A smart device for label-free and real-time detection of gene point mutation-related diseases was developed based on the high dark phase contrast of vapor condensation. The main components of the device included a Peltier cooler and a mini PC board for image processing. Heat from the hot side of the Peltier cooler causes the fluid in a copper chamber to evaporate, and the vapor condenses on the surface of a microarray chip placed on the cold side of the cooler. The high dark phase contrast of vapor condensation relative to the analytes on the microarray chip was explored. Combined with rolling circle amplification, the device visualizes less-to-more hydrophilic transitions caused by gene trapping and DNA amplification. A lung cancer gene point mutation was analysed, proving the high selectivity and multiplex analysis capability of this low-cost device.",
author = "Junqi Zhang and Rongxin Fu and Liping Xie and Qi Li and Wenhan Zhou and Ruliang Wang and Jiancheng Ye and Dong Wang and Ning Xue and Xue Lin and Ying Lu and Guoliang Huang",
note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",
year = "2015",
month = jul,
day = "28",
doi = "10.1039/c5lc00488h",
language = "English",
volume = "15",
pages = "3891--3896",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "19",
}
Zhang, J, Fu, R, Xie, L, Li, Q, Zhou, W, Wang, R, Ye, J, Wang, D, Xue, N, Lin, X, Lu, Y & Huang, G 2015, 'A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation', Lab on a Chip, 卷 15, 号码 19, 页码 3891-3896. https://doi.org/10.1039/c5lc00488h
A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation. / Zhang, Junqi
; Fu, Rongxin; Xie, Liping 等.
在:
Lab on a Chip, 卷 15, 号码 19, 28.07.2015, 页码 3891-3896.
科研成果: 期刊稿件 › 文章 › 同行评审
TY - JOUR
T1 - A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation
AU - Zhang, Junqi
AU - Fu, Rongxin
AU - Xie, Liping
AU - Li, Qi
AU - Zhou, Wenhan
AU - Wang, Ruliang
AU - Ye, Jiancheng
AU - Wang, Dong
AU - Xue, Ning
AU - Lin, Xue
AU - Lu, Ying
AU - Huang, Guoliang
N1 - Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2015/7/28
Y1 - 2015/7/28
N2 - A smart device for label-free and real-time detection of gene point mutation-related diseases was developed based on the high dark phase contrast of vapor condensation. The main components of the device included a Peltier cooler and a mini PC board for image processing. Heat from the hot side of the Peltier cooler causes the fluid in a copper chamber to evaporate, and the vapor condenses on the surface of a microarray chip placed on the cold side of the cooler. The high dark phase contrast of vapor condensation relative to the analytes on the microarray chip was explored. Combined with rolling circle amplification, the device visualizes less-to-more hydrophilic transitions caused by gene trapping and DNA amplification. A lung cancer gene point mutation was analysed, proving the high selectivity and multiplex analysis capability of this low-cost device.
AB - A smart device for label-free and real-time detection of gene point mutation-related diseases was developed based on the high dark phase contrast of vapor condensation. The main components of the device included a Peltier cooler and a mini PC board for image processing. Heat from the hot side of the Peltier cooler causes the fluid in a copper chamber to evaporate, and the vapor condenses on the surface of a microarray chip placed on the cold side of the cooler. The high dark phase contrast of vapor condensation relative to the analytes on the microarray chip was explored. Combined with rolling circle amplification, the device visualizes less-to-more hydrophilic transitions caused by gene trapping and DNA amplification. A lung cancer gene point mutation was analysed, proving the high selectivity and multiplex analysis capability of this low-cost device.
UR - http://www.scopus.com/inward/record.url?scp=84941788741&partnerID=8YFLogxK
U2 - 10.1039/c5lc00488h
DO - 10.1039/c5lc00488h
M3 - Article
C2 - 26266399
AN - SCOPUS:84941788741
SN - 1473-0197
VL - 15
SP - 3891
EP - 3896
JO - Lab on a Chip
JF - Lab on a Chip
IS - 19
ER -
Zhang J, Fu R, Xie L, Li Q, Zhou W, Wang R 等. A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation. Lab on a Chip. 2015 7月 28;15(19):3891-3896. doi: 10.1039/c5lc00488h
