TY - GEN
T1 - A sensitive determination of 1-acetyl-6,7-dihydroxyl-1,2,3,4-tetrahydroisoquinoline (ADTIQ) in rat substantia nigra by multiple reaction monitoring (MRM)
AU - Xie, Bing Jie
AU - Wu, Han Yan
AU - Ullah, Kaleem
AU - Peng, Lei
AU - Qing, Hong
AU - Deng, Yu Lin
N1 - Publisher Copyright:
© 2015 Taylor & Francis Group, London.
PY - 2015
Y1 - 2015
N2 - It was considered that 1-acetyl-6,7-dihydroxyl-1,2,3,4-Tetrahydroisoquinoline (ADTIQ), and its precursor methylglyoxal were closely related to glucose metabolism. ADTIQ was similar in structure with catecholamine isoquinolines (CAIQs). Therefore, ADTIQ was thought to be related to diabetes and Parkinson’s Disease (PD). The quantitation of ADTIQ in a biological sample could play an important role in detection of diabetic-PD. Herein, a sensitive and precise method was established to quantify ADTIQ by LC/MS/MS in Multiple Reaction Monitoring (MRM) mode. The [M + H] + precursor/product ions were 208.1/190.1. The linear range of calibration curve was from 0.01-2 nM, and the lower limit of ADTIQ quantitation is 0.002 nM. Recoveries for biological sample were 137-162% with acceptable inter-day, intra-day precision and accuracy. Furthermore, the assay method was used for the determination of ADTIQ in rat substantia nigra.
AB - It was considered that 1-acetyl-6,7-dihydroxyl-1,2,3,4-Tetrahydroisoquinoline (ADTIQ), and its precursor methylglyoxal were closely related to glucose metabolism. ADTIQ was similar in structure with catecholamine isoquinolines (CAIQs). Therefore, ADTIQ was thought to be related to diabetes and Parkinson’s Disease (PD). The quantitation of ADTIQ in a biological sample could play an important role in detection of diabetic-PD. Herein, a sensitive and precise method was established to quantify ADTIQ by LC/MS/MS in Multiple Reaction Monitoring (MRM) mode. The [M + H] + precursor/product ions were 208.1/190.1. The linear range of calibration curve was from 0.01-2 nM, and the lower limit of ADTIQ quantitation is 0.002 nM. Recoveries for biological sample were 137-162% with acceptable inter-day, intra-day precision and accuracy. Furthermore, the assay method was used for the determination of ADTIQ in rat substantia nigra.
UR - http://www.scopus.com/inward/record.url?scp=84940727716&partnerID=8YFLogxK
U2 - 10.1201/b18418-103
DO - 10.1201/b18418-103
M3 - Conference contribution
AN - SCOPUS:84940727716
T3 - Medicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
SP - 565
EP - 570
BT - Medicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
A2 - Wang, Mings
PB - CRC Press/Balkema
T2 - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
Y2 - 16 August 2014 through 17 August 2014
ER -
