A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

Fanghao Shi; Fei Jia; Weikai Zhang; Zewen Wei

doi:10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

Fanghao Shi, Fei Jia, Weikai Zhang, Zewen Wei^*

^*此作品的通讯作者

医学技术学院

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

摘要

Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

源语言	英语
主期刊名	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
出版商	Institute of Electrical and Electronics Engineers Inc.
页	1010-1013
页数	4
ISBN（电子版）	9781728135809
DOI	https://doi.org/10.1109/MEMS46641.2020.9056350
出版状态	已出版 - 1月 2020
活动	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, 加拿大期限: 18 1月 2020 → 22 1月 2020

出版系列

姓名	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
卷	2020-January
ISSN（印刷版）	1084-6999

会议

会议	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
国家/地区	加拿大
市	Vancouver
时期	18/01/20 → 22/01/20

访问文件

10.1109/MEMS46641.2020.9056350

其它文件与链接

链接到 Scopus 的出版物

引用此

Shi, F., Jia, F., Zhang, W., & Wei, Z. (2020). A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. 在 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (页码 1010-1013). 文章 9056350 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); 卷 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056350

Shi, Fanghao ; Jia, Fei ; Zhang, Weikai 等. / A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. 页码 1010-1013 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{1c7d083d62ac4fbf802768e33cff5abf,

title = "A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level",

abstract = "Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.",

keywords = "Circulating tumor cells (CTCs), single cell RNA sequencing",

author = "Fanghao Shi and Fei Jia and Weikai Zhang and Zewen Wei",

note = "Publisher Copyright: {\textcopyright} 2020 IEEE.; 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 ; Conference date: 18-01-2020 Through 22-01-2020",

year = "2020",

month = jan,

doi = "10.1109/MEMS46641.2020.9056350",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "1010--1013",

booktitle = "33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020",

address = "United States",

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Shi, F, Jia, F, Zhang, W & Wei, Z 2020, A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. 在 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020., 9056350, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 卷 2020-January, Institute of Electrical and Electronics Engineers Inc., 页码 1010-1013, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020, Vancouver, 加拿大, 18/01/20. https://doi.org/10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. / Shi, Fanghao; Jia, Fei; Zhang, Weikai 等.
33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. 页码 1010-1013 9056350 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); 卷 2020-January).

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

TY - GEN

T1 - A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

AU - Shi, Fanghao

AU - Jia, Fei

AU - Zhang, Weikai

AU - Wei, Zewen

PY - 2020/1

Y1 - 2020/1

N2 - Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

AB - Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

KW - Circulating tumor cells (CTCs)

KW - single cell RNA sequencing

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U2 - 10.1109/MEMS46641.2020.9056350

DO - 10.1109/MEMS46641.2020.9056350

M3 - Conference contribution

AN - SCOPUS:85083186855

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 1010

EP - 1013

BT - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020

Y2 - 18 January 2020 through 22 January 2020

ER -

Shi F, Jia F, Zhang W, Wei Z. A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. 在 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc. 2020. 页码 1010-1013. 9056350. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

摘要

出版系列

会议

访问文件

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引用此