TY - GEN
T1 - A method to improve the universality of siRNA design rules based on siRNA efficiency distribution
AU - Wang, Dongfang
AU - Chen, Xiang
AU - Zhang, Fa
AU - Zhang, Peizhuo
AU - Liu, Guohua
AU - Guo, Xin
PY - 2008
Y1 - 2008
N2 - Effectual siRNA design is a pivotal question in RNAi research. However existing siRNA design guidelines are not very consistent with each other, and many have the limitation of universality, that is, many guidelines usually present poor predicting capability on independent siRNA datasets. In order to explore high universal siRNA design rules, first we collected as many siRNA valid data on various genes and species as possible and sampled the training datasets according to the distribution of siRNA efficiency in a nature state based on statistic principle. Then we performed two sets of analysis process in parallel adopting different statistical methods to explore the rules for functional siRNA design. Finally we tested the rules on internal and external test set. Test results indicated the functional siRNA design rules obtained by our analysis method had good predicting capability and universality.
AB - Effectual siRNA design is a pivotal question in RNAi research. However existing siRNA design guidelines are not very consistent with each other, and many have the limitation of universality, that is, many guidelines usually present poor predicting capability on independent siRNA datasets. In order to explore high universal siRNA design rules, first we collected as many siRNA valid data on various genes and species as possible and sampled the training datasets according to the distribution of siRNA efficiency in a nature state based on statistic principle. Then we performed two sets of analysis process in parallel adopting different statistical methods to explore the rules for functional siRNA design. Finally we tested the rules on internal and external test set. Test results indicated the functional siRNA design rules obtained by our analysis method had good predicting capability and universality.
KW - Design rules
KW - Effectual siRNA
KW - Efficiency disrutution
KW - Internal and external test set
KW - Universality
UR - http://www.scopus.com/inward/record.url?scp=62449307897&partnerID=8YFLogxK
U2 - 10.1109/ISISE.2008.284
DO - 10.1109/ISISE.2008.284
M3 - Conference contribution
AN - SCOPUS:62449307897
SN - 9780769534947
T3 - 2008 International Symposium on Information Science and Engineering, ISISE 2008
SP - 84
EP - 87
BT - 2008 International Symposium on Information Science and Engineering, ISISE 2008
T2 - 2008 International Symposium on Information Science and Engineering, ISISE 2008
Y2 - 20 December 2008 through 22 December 2008
ER -
