A high performance continuous electroporation chip

Zewen Wei, Huang Huang, Mengxi Wu, Zicai Liang, Wei Wang, Zhihong Li*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

1 引用 (Scopus)

摘要

Electroporation is an effective method for delivery of biological components into mammalian cells and is of great importance for modern life science. Here we report a novel continuous electroporation chip with great performance. Integration of the microfluid channel, micromachined Au electrodes and hydrodynamic focusing enables high-throughput and high-efficiency electroporation. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent transfection rate (90%), cell viability (60%) and cell treating speed (1.5×10 cells per minute), all much higher than those reported in literature.

源语言英语
主期刊名14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
1622-1624
页数3
出版状态已出版 - 2010
已对外发布
活动14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, 荷兰
期限: 3 10月 20107 10月 2010

出版系列

姓名14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
3

会议

会议14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
国家/地区荷兰
Groningen
时期3/10/107/10/10

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