TY - GEN
T1 - A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis
AU - Li, Xueming
AU - Wei, Zewen
AU - Zhao, Deyao
AU - Yan, Hao
AU - Liang, Zicai
AU - Li, Zhihong
PY - 2012
Y1 - 2012
N2 - Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.
AB - Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.
UR - http://www.scopus.com/inward/record.url?scp=84860474881&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2012.6170353
DO - 10.1109/MEMSYS.2012.6170353
M3 - Conference contribution
AN - SCOPUS:84860474881
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 993
EP - 996
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -