TY - JOUR
T1 - 毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用
AU - Li, Yu Juan
AU - Sun, Xin Xin
AU - Li, Pan Pan
AU - Ou, Wan Lu
AU - Qu, Feng
N1 - Publisher Copyright:
© 2019, Editorial Department of Transaction of Beijing Institute of Technology. All right reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73×104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98×104, 1.83×104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.
AB - Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73×104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98×104, 1.83×104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.
KW - Active molecules
KW - Aptamer
KW - B-Thr
KW - Capillary zone electrophoresis
KW - Dragon's blood
UR - http://www.scopus.com/inward/record.url?scp=85063295363&partnerID=8YFLogxK
U2 - 10.15918/j.tbit1001-0645.2019.01.018
DO - 10.15918/j.tbit1001-0645.2019.01.018
M3 - 文章
AN - SCOPUS:85063295363
SN - 1001-0645
VL - 39
SP - 106
EP - 110
JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
IS - 1
ER -