TY - JOUR
T1 - 分子印迹磁性荧光复合微球的制备及其性能研究
AU - Luo, Ai Qin
AU - Chen, Wei Jie
AU - Zhang, Xin
AU - Liu, Hong Yang
AU - Li, Yuan Yuan
AU - Sun, Li Quan
N1 - Publisher Copyright:
© 2020, Editorial Department of Transaction of Beijing Institute of Technology. All right reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Rapid and effective separation of protein in complex biological samples is very important in the field of life science research, such as biological separation, protein purification and so on. In this study, Fe3O4MNP-ZnSQDs were used as carriers, and lysozyme was chosen as template protein. The molecularly imprinted polymer was coated on the surface of the Fe3O4MNP-ZnSQDs to form magnetic fluorescent micro-spheres via surface imprinting process. The experiment results show that Fe3O4MNP-ZnSQD@MIPs possesses better dispersibility, uniform particle size and strong fluorescence emission, which can be rapidly separated under external magnetic field. Under optimum conditions, the adsorption equilibrium of Fe3O4MNP-ZnSQD@MIPs can be achieved in 15 minutes, and the maximum adsorption capacity is 645.76 mg•g-1, the saturation magnetic intensity is 40 emu•g-1. Moreover, the prepared Fe3O4MNP-ZnSQD@MIPs possesses adsorption selectivity, and the imprinting factor is 2.15. The advantages of the prepared Fe3O4MNP-ZnSQD@MIPs include low cost, fast analysis process, convenience and large adsorption capacity. It can be used for rapid separation and purification of lysozyme in complex biological sample.
AB - Rapid and effective separation of protein in complex biological samples is very important in the field of life science research, such as biological separation, protein purification and so on. In this study, Fe3O4MNP-ZnSQDs were used as carriers, and lysozyme was chosen as template protein. The molecularly imprinted polymer was coated on the surface of the Fe3O4MNP-ZnSQDs to form magnetic fluorescent micro-spheres via surface imprinting process. The experiment results show that Fe3O4MNP-ZnSQD@MIPs possesses better dispersibility, uniform particle size and strong fluorescence emission, which can be rapidly separated under external magnetic field. Under optimum conditions, the adsorption equilibrium of Fe3O4MNP-ZnSQD@MIPs can be achieved in 15 minutes, and the maximum adsorption capacity is 645.76 mg•g-1, the saturation magnetic intensity is 40 emu•g-1. Moreover, the prepared Fe3O4MNP-ZnSQD@MIPs possesses adsorption selectivity, and the imprinting factor is 2.15. The advantages of the prepared Fe3O4MNP-ZnSQD@MIPs include low cost, fast analysis process, convenience and large adsorption capacity. It can be used for rapid separation and purification of lysozyme in complex biological sample.
KW - Magnetic fluorescent nano-spheres
KW - Molecularly imprinted technology
KW - Protein separation
UR - http://www.scopus.com/inward/record.url?scp=85094912587&partnerID=8YFLogxK
U2 - 10.15918/j.tbit1001-0645.2019.112
DO - 10.15918/j.tbit1001-0645.2019.112
M3 - 文章
AN - SCOPUS:85094912587
SN - 1001-0645
VL - 40
SP - 1126
EP - 1132
JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
IS - 10
ER -