分子印迹磁性荧光复合微球的制备及其性能研究

Ai Qin Luo, Wei Jie Chen, Xin Zhang, Hong Yang Liu, Yuan Yuan Li, Li Quan Sun

科研成果: 期刊稿件文章同行评审

1 引用 (Scopus)

摘要

Rapid and effective separation of protein in complex biological samples is very important in the field of life science research, such as biological separation, protein purification and so on. In this study, Fe3O4MNP-ZnSQDs were used as carriers, and lysozyme was chosen as template protein. The molecularly imprinted polymer was coated on the surface of the Fe3O4MNP-ZnSQDs to form magnetic fluorescent micro-spheres via surface imprinting process. The experiment results show that Fe3O4MNP-ZnSQD@MIPs possesses better dispersibility, uniform particle size and strong fluorescence emission, which can be rapidly separated under external magnetic field. Under optimum conditions, the adsorption equilibrium of Fe3O4MNP-ZnSQD@MIPs can be achieved in 15 minutes, and the maximum adsorption capacity is 645.76 mg•g-1, the saturation magnetic intensity is 40 emu•g-1. Moreover, the prepared Fe3O4MNP-ZnSQD@MIPs possesses adsorption selectivity, and the imprinting factor is 2.15. The advantages of the prepared Fe3O4MNP-ZnSQD@MIPs include low cost, fast analysis process, convenience and large adsorption capacity. It can be used for rapid separation and purification of lysozyme in complex biological sample.

投稿的翻译标题Preparation and Properties of Molecularly Imprinted Magnetic Fluorescent Composite Microspheres
源语言繁体中文
页(从-至)1126-1132
页数7
期刊Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
40
10
DOI
出版状态已出版 - 1 10月 2020

关键词

  • Magnetic fluorescent nano-spheres
  • Molecularly imprinted technology
  • Protein separation

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