Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope

Muyue Zhai; Xiaoshuai Huang; Heng Mao; Qiudong Zhu; Shanshan Wang

doi:10.1007/978-3-319-91659-0_34

Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope

Muyue Zhai, Xiaoshuai Huang, Heng Mao, Qiudong Zhu, Shanshan Wang^*

^*Corresponding author for this work

School of Optics and Photonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.

Original language	English
Title of host publication	Proceedings of the International Conference on Sensing and Imaging
Editors	Eric Todd Quinto, Nathan Ida, Alfred K. Louis, Ming Jiang
Publisher	Springer Verlag
Pages	411-419
Number of pages	9
ISBN (Print)	9783319916583
DOIs	https://doi.org/10.1007/978-3-319-91659-0_34
Publication status	Published - 2019
Event	International Conference on Sensing and Imaging, ICSI 2017 - Chengdu, China Duration: 5 Jun 2017 → 7 Jun 2017

Publication series

Name	Lecture Notes in Electrical Engineering
Volume	506
ISSN (Print)	1876-1100
ISSN (Electronic)	1876-1119

Conference

Conference	International Conference on Sensing and Imaging, ICSI 2017
Country/Territory	China
City	Chengdu
Period	5/06/17 → 7/06/17

Keywords

Electrically tunable lens
High axial resolution
Large field of view
Light sheet fluorescence microscope

Access to Document

10.1007/978-3-319-91659-0_34

Cite this

Zhai, M., Huang, X., Mao, H., Zhu, Q., & Wang, S. (2019). Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope. In E. T. Quinto, N. Ida, A. K. Louis, & M. Jiang (Eds.), Proceedings of the International Conference on Sensing and Imaging (pp. 411-419). (Lecture Notes in Electrical Engineering; Vol. 506). Springer Verlag. https://doi.org/10.1007/978-3-319-91659-0_34

Zhai, Muyue ; Huang, Xiaoshuai ; Mao, Heng et al. / Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope. Proceedings of the International Conference on Sensing and Imaging. editor / Eric Todd Quinto ; Nathan Ida ; Alfred K. Louis ; Ming Jiang. Springer Verlag, 2019. pp. 411-419 (Lecture Notes in Electrical Engineering).

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title = "Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope",

abstract = "Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.",

keywords = "Electrically tunable lens, High axial resolution, Large field of view, Light sheet fluorescence microscope",

author = "Muyue Zhai and Xiaoshuai Huang and Heng Mao and Qiudong Zhu and Shanshan Wang",

note = "Publisher Copyright: {\textcopyright} Springer International Publishing AG, part of Springer Nature 2019.; International Conference on Sensing and Imaging, ICSI 2017 ; Conference date: 05-06-2017 Through 07-06-2017",

year = "2019",

doi = "10.1007/978-3-319-91659-0_34",

language = "English",

isbn = "9783319916583",

series = "Lecture Notes in Electrical Engineering",

publisher = "Springer Verlag",

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address = "Germany",

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Zhai, M, Huang, X, Mao, H, Zhu, Q & Wang, S 2019, Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope. in ET Quinto, N Ida, AK Louis & M Jiang (eds), Proceedings of the International Conference on Sensing and Imaging. Lecture Notes in Electrical Engineering, vol. 506, Springer Verlag, pp. 411-419, International Conference on Sensing and Imaging, ICSI 2017, Chengdu, China, 5/06/17. https://doi.org/10.1007/978-3-319-91659-0_34

Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope. / Zhai, Muyue; Huang, Xiaoshuai; Mao, Heng et al.
Proceedings of the International Conference on Sensing and Imaging. ed. / Eric Todd Quinto; Nathan Ida; Alfred K. Louis; Ming Jiang. Springer Verlag, 2019. p. 411-419 (Lecture Notes in Electrical Engineering; Vol. 506).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

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AU - Zhai, Muyue

AU - Huang, Xiaoshuai

AU - Mao, Heng

AU - Zhu, Qiudong

AU - Wang, Shanshan

N1 - Publisher Copyright: © Springer International Publishing AG, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.

AB - Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.

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KW - High axial resolution

KW - Large field of view

KW - Light sheet fluorescence microscope

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Zhai M, Huang X, Mao H, Zhu Q, Wang S. Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope. In Quinto ET, Ida N, Louis AK, Jiang M, editors, Proceedings of the International Conference on Sensing and Imaging. Springer Verlag. 2019. p. 411-419. (Lecture Notes in Electrical Engineering). doi: 10.1007/978-3-319-91659-0_34

Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope

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