Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode

Jieling Qin; Misuk Cho; Youngkwan Lee

doi:10.1021/acs.analchem.9b02266

Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode

Jieling Qin, Misuk Cho^*, Youngkwan Lee

^*Corresponding author for this work

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

A highly sensitive electrochemical impedance sensor for amyloid beta oligomer (AβO) was fabricated using a cellular prion protein (PrP^C) bioreceptor linked with poly(thiophene-3-acetic acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene) embedded with gold nanoparticles was employed to provide high electrical conductivity and a large surface area. The sensing performace was investigated in terms of sensitivity and detection range. The fabricated sensor exhibited extremely low detection limit at a subfemtomolar level with a wide detection range from 10^-8 to 10⁴ nM and its utility was established in mice infected with Alzheimer's disease (AD). The developed AβO sensor could be utilized for early diagnosis of AD.

Original language	English
Pages (from-to)	11259-11265
Number of pages	7
Journal	Analytical Chemistry
Volume	91
Issue number	17
DOIs	https://doi.org/10.1021/acs.analchem.9b02266
Publication status	Published - 3 Sept 2019
Externally published	Yes

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10.1021/acs.analchem.9b02266

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title = "Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode",

abstract = "A highly sensitive electrochemical impedance sensor for amyloid beta oligomer (AβO) was fabricated using a cellular prion protein (PrPC) bioreceptor linked with poly(thiophene-3-acetic acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene) embedded with gold nanoparticles was employed to provide high electrical conductivity and a large surface area. The sensing performace was investigated in terms of sensitivity and detection range. The fabricated sensor exhibited extremely low detection limit at a subfemtomolar level with a wide detection range from 10-8 to 104 nM and its utility was established in mice infected with Alzheimer's disease (AD). The developed AβO sensor could be utilized for early diagnosis of AD.",

author = "Jieling Qin and Misuk Cho and Youngkwan Lee",

note = "Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",

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doi = "10.1021/acs.analchem.9b02266",

language = "English",

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Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode. / Qin, Jieling; Cho, Misuk; Lee, Youngkwan.
In: Analytical Chemistry, Vol. 91, No. 17, 03.09.2019, p. 11259-11265.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode

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AU - Cho, Misuk

AU - Lee, Youngkwan

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Y1 - 2019/9/3

N2 - A highly sensitive electrochemical impedance sensor for amyloid beta oligomer (AβO) was fabricated using a cellular prion protein (PrPC) bioreceptor linked with poly(thiophene-3-acetic acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene) embedded with gold nanoparticles was employed to provide high electrical conductivity and a large surface area. The sensing performace was investigated in terms of sensitivity and detection range. The fabricated sensor exhibited extremely low detection limit at a subfemtomolar level with a wide detection range from 10-8 to 104 nM and its utility was established in mice infected with Alzheimer's disease (AD). The developed AβO sensor could be utilized for early diagnosis of AD.

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JO - Analytical Chemistry

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Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode

Abstract

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