The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

Li Qian; Qu Feng; Hongmei; Gong; Deng Yulin

doi:10.1109/ICCME.2007.4382037

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

Li Qian, Qu Feng^*, Hongmei, Gong, Deng Yulin

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

Original language	English
Title of host publication	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Pages	1698-1701
Number of pages	4
DOIs	https://doi.org/10.1109/ICCME.2007.4382037
Publication status	Published - 2007
Event	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, China Duration: 23 May 2007 → 27 May 2007

Publication series

Name	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Conference

Conference	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Country/Territory	China
City	Beijing
Period	23/05/07 → 27/05/07

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10.1109/ICCME.2007.4382037

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Qian, L., Feng, Q., Hongmei, Gong, & Yulin, D. (2007). The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. In 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 (pp. 1698-1701). Article 4382037 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). https://doi.org/10.1109/ICCME.2007.4382037

@inproceedings{c9eb648281b54389ad389bd9f6a31fea,

title = "The optimization of proteins assay under extreme acidic condition with capillary electrophoresis",

abstract = "Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.",

author = "Li Qian and Qu Feng and Hongmei and Gong and Deng Yulin",

year = "2007",

doi = "10.1109/ICCME.2007.4382037",

language = "English",

isbn = "1424410789",

series = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

pages = "1698--1701",

booktitle = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

note = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 ; Conference date: 23-05-2007 Through 27-05-2007",

}

Qian, L, Feng, Q, Hongmei, Gong, & Yulin, D 2007, The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. in 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007., 4382037, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, pp. 1698-1701, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, Beijing, China, 23/05/07. https://doi.org/10.1109/ICCME.2007.4382037

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. / Qian, Li; Feng, Qu; Hongmei et al.
2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. p. 1698-1701 4382037 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

AU - Qian, Li

AU - Feng, Qu

AU - Hongmei,

AU - Gong,

AU - Yulin, Deng

PY - 2007

Y1 - 2007

N2 - Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

AB - Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

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U2 - 10.1109/ICCME.2007.4382037

DO - 10.1109/ICCME.2007.4382037

M3 - Conference contribution

AN - SCOPUS:48149115329

SN - 1424410789

SN - 9781424410781

T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

SP - 1698

EP - 1701

BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Y2 - 23 May 2007 through 27 May 2007

ER -

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

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