The novel route to prepare immobilized macrocyclic compound on 6-OH of chitosan with good solubility and prime study on its applications

β-cyclodextrin (β-CD) immobilization of a chitosan derivate on C₆-OH (CTS-6-CD) with good solubility was prepared through a novel method. During the process the amino group of the chitosan was protected by phthalic anhydride and deprotected in the hydrazine hydrate solution. β-CD was immobilized onto the C₆ position of chitosan by a nucleophilic displacement reaction. The prepared derivate can be dissolved in hydrazine hydrate (v:v = 1:1), ethylenediamine/ethanol (v:v = 1:1), 1% aqueous acetic acid, DMSO and DMAc. The application of the 6-OH cyclodextrin-immobilized chitosan derivatives in an electrochemical biosensor was examined in a preliminarily manner.