Abstract
An immobilized pH gradient method had been developed for proteins separation by two-step capillary isoelectric focusing based on chemical bonding. The double functional reagent, N,N′-carbonyldiimidazole was introduced in the capillary as a linkage in the immobilization reactions. Moreover, the commercial ampholytes (CAs) were distributed according to their pI values in the condition of electric field, and then connected with N,N′- carbonyldiimidazole by chemical bonding to realize the immobilized pH gradient in capillary. A test sample containing a protein mixture of hemoglobin (Hb), bovine serum albumin (BSA) and pepsin was separated by using this method.
| Original language | English |
|---|---|
| Pages (from-to) | 461-464 |
| Number of pages | 4 |
| Journal | Chemistry Bulletin / Huaxue Tongbao |
| Volume | 71 |
| Issue number | 6 |
| Publication status | Published - 2008 |
Keywords
- Capillary isoelectric focusing
- Immobilized pH gradient
- N,N′-carbonyldiimidazole
- Protein separation