TY - GEN
T1 - Synthesis and characterization of a novel natural macromolecule antibacterial material
AU - Jianhua, Sun
AU - Nannan, Zheng
AU - Rongji, Dai
AU - Weiwei, Meng
AU - Yulin, Deng
PY - 2009
Y1 - 2009
N2 - A novel natural macromolecule Antibacterial material was synthesized by reaction with carboxymethylchitosan, decanoyldimethylammonium and epichlorohydrin. Long chain quaternary ammonium (OGTA) was prepared by reaction with decanoyldimethylammonium and epichlorohydrin, using isopropylalcohol as solvent, at 55°C for 6 hours, and vacuum distillation. The yield was 87 percent. With 0.5g CMCTS and 2g OGTA as initiative materials, 0.5g sodium hydroxide regulating the value of PH, reacting at 30 ml Nmethylpyrrolidone for 8 hours and stirring quickly at 70°C , carboxymethylchitosan derivative (CMCTS-OGTA) was obtained. The yield was 95 percent. Characterization of the product by IR spectrum, 1H-NMR spectrum and element analysis revealed that the experiments had got the wanted product. Bacteriostatic activity and antibacterial activity were characterized by the greatest bacteriostatic circle method, which indicated that CMCTS-OGTA definitely had an inhibitory activity against S. aureus and bacillus subtilis. Comparation of the Bacteriostatic activities of CMCTS-OGTA and CMCTS suggested that CMCTS-OGTA had more perfect Antibacterial capability. The lowest effectively antibacterial concentrations against Bacillus coli and S. aureus were also determined.
AB - A novel natural macromolecule Antibacterial material was synthesized by reaction with carboxymethylchitosan, decanoyldimethylammonium and epichlorohydrin. Long chain quaternary ammonium (OGTA) was prepared by reaction with decanoyldimethylammonium and epichlorohydrin, using isopropylalcohol as solvent, at 55°C for 6 hours, and vacuum distillation. The yield was 87 percent. With 0.5g CMCTS and 2g OGTA as initiative materials, 0.5g sodium hydroxide regulating the value of PH, reacting at 30 ml Nmethylpyrrolidone for 8 hours and stirring quickly at 70°C , carboxymethylchitosan derivative (CMCTS-OGTA) was obtained. The yield was 95 percent. Characterization of the product by IR spectrum, 1H-NMR spectrum and element analysis revealed that the experiments had got the wanted product. Bacteriostatic activity and antibacterial activity were characterized by the greatest bacteriostatic circle method, which indicated that CMCTS-OGTA definitely had an inhibitory activity against S. aureus and bacillus subtilis. Comparation of the Bacteriostatic activities of CMCTS-OGTA and CMCTS suggested that CMCTS-OGTA had more perfect Antibacterial capability. The lowest effectively antibacterial concentrations against Bacillus coli and S. aureus were also determined.
KW - Bacteriostatic activity
KW - Carboxymethylchitosan
KW - Quaternary ammonium
UR - http://www.scopus.com/inward/record.url?scp=72749103962&partnerID=8YFLogxK
U2 - 10.1109/ICBBE.2009.5163550
DO - 10.1109/ICBBE.2009.5163550
M3 - Conference contribution
AN - SCOPUS:72749103962
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -