TY - JOUR
T1 - Surface plasmon resonance sensor for femtomolar detection of testosterone with water-compatible macroporous molecularly imprinted film
AU - Zhang, Qingwen
AU - Jing, Lijing
AU - Wang, Yi
AU - Zhang, Jinling
AU - Ren, Yamin
AU - Wang, Yang
AU - Wei, Tianxin
AU - Liedberg, Bo
PY - 2014/10/15
Y1 - 2014/10/15
N2 - A novel water-compatible macroporous molecularly imprinted film (MIF) has been developed for rapid, sensitive, and label-free detection of small molecule testosterone in urine. The MIF was synthesized by photo copolymerization of monomers (methacrylic acid [MAA] and 2-hydroxyethyl methacrylate [HEMA]), cross-linker (ethylene glycol dimethacrylate, EGDMA), and polystyrene nanoparticles (PS NPs) in combination with template testosterone molecules. The PS NPs and template molecules were subsequently removed to form an MIF with macroporous structures and the specific recognition sites of testosterone. Incubation of artificial urine and human urine on the MIF and the non-imprinted film (NIF), respectively, indicated undetectable nonspecific adsorption. Accordingly, the MIF was applied on a surface plasmon resonance (SPR) sensor for the detection of testosterone in phosphate-buffered saline (PBS) and artificial urine with a limit of detection (LOD) down to 10-15 g/ml. To the best of our knowledge, the LOD is considered as one of the lowest among the SPR sensors for the detection of small molecules. The control experiments performed with analogue molecules such as progesterone and estradiol demonstrated the good selectivity of this MIF for sensing testosterone. Furthermore, this MIF-based SPR sensor shows high stability and reproducibility over 8 months of storage at room temperature, which is more robust than protein-based biosensors.
AB - A novel water-compatible macroporous molecularly imprinted film (MIF) has been developed for rapid, sensitive, and label-free detection of small molecule testosterone in urine. The MIF was synthesized by photo copolymerization of monomers (methacrylic acid [MAA] and 2-hydroxyethyl methacrylate [HEMA]), cross-linker (ethylene glycol dimethacrylate, EGDMA), and polystyrene nanoparticles (PS NPs) in combination with template testosterone molecules. The PS NPs and template molecules were subsequently removed to form an MIF with macroporous structures and the specific recognition sites of testosterone. Incubation of artificial urine and human urine on the MIF and the non-imprinted film (NIF), respectively, indicated undetectable nonspecific adsorption. Accordingly, the MIF was applied on a surface plasmon resonance (SPR) sensor for the detection of testosterone in phosphate-buffered saline (PBS) and artificial urine with a limit of detection (LOD) down to 10-15 g/ml. To the best of our knowledge, the LOD is considered as one of the lowest among the SPR sensors for the detection of small molecules. The control experiments performed with analogue molecules such as progesterone and estradiol demonstrated the good selectivity of this MIF for sensing testosterone. Furthermore, this MIF-based SPR sensor shows high stability and reproducibility over 8 months of storage at room temperature, which is more robust than protein-based biosensors.
KW - Macroporous film
KW - Molecularly imprinted film (MIF)
KW - Sensor
KW - Surface plasmon resonance (SPR)
KW - Testosterone
KW - Water-compatible
UR - http://www.scopus.com/inward/record.url?scp=84905159093&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2014.06.014
DO - 10.1016/j.ab.2014.06.014
M3 - Article
C2 - 24991687
AN - SCOPUS:84905159093
SN - 0003-2697
VL - 463
SP - 7
EP - 14
JO - Analytical Biochemistry
JF - Analytical Biochemistry
ER -