Surface plasmon resonance sensor chips for the recognition of bovine serum albumin via electropolymerized molecularly imprinted polymers

In this paper, a surface plasmon resonance (SPR) sensor chip for detection of bovine serum album (BSA) was prepared by electropolymerization of 3-aminophenylboronic acid (3-APBA) based on molecularly imprinted polymer (MIP) technique. The surface morphology of MIP and non-imprinted (NIP) films were characterized by scanning electroscopy (SEM). SEM images exhibited nanoscale cavities formed on the MIP films surface homogeneously due to the removal of BSA templates. The effects of pH, ion strength of rebinding BSA, the specific binding and selective recognition were studied for MIP films. Results indicated that the BSA-imprinted films exhibited a good adsorption of template protein (0.02-0.8 mg/mL) in 0.05 mol/L sodium phosphate buffer at pH 5.0 with the limit of detection (LOD) of 0.02 mg/mL.