Abstract
Aim: The cell injury mechanism of manganese acetate was investigated using PC12. Methods: Cultured PC12 cells were exposed to Mn (Ac)3 for 24 hours. The survival rate of PC12 cells was determined by MTT assay. Hoechst 33258 and DNA ladder were performed to monitor the apoptotic cells. HPLC-ECD was used to measure the levels of hydroxyl radical and catechol isoquinoline in cells. Lipid peroxidant product MDA was also measured. Results: The viability of PC12 cells decreased as the Mn(Ac)3 concentration increased. The manganese treatment could induce PC12 cell apoptosis. The level of MDA and hydroxyl radical increased compared with that of the control (P < 0. 05). DA levels decreased significantly in manganese acetate treated PC12 cells (P < 0. 05). Sal and NMSal levels increased with the increment of manganese concentration. Conclusion: Excessive manganese can induce the high level of oxidative stress in PC12 cells, and the generation of catechol isoquinolines may be toxic to PC12 cells, which may be a way in the manganese acetate induced injury of PC12 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1574-1578 |
| Number of pages | 5 |
| Journal | Chinese Pharmacological Bulletin |
| Volume | 24 |
| Issue number | 12 |
| Publication status | Published - Dec 2008 |
Keywords
- Injury
- Manganese acetate
- Oxidative stress
- PC12 cells