Abstract
A method was used to investigated S-Catechol-O-Methyltransferase activity(COMT) with 3,4-dihydroxybenzoicacid as the substrate C18 column (150mm ×4.6 mm) at 25°C. The mobile phase consisted of methanol and 0.07 % acetic acid aqueous solution with a flow rate of 1.0mL/min at 254nm. The optimal pH value was 8 and temperature was 45°C for S-COMT specific activity.
| Original language | English |
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| Title of host publication | ICBBT 2010 - 2010 International Conference on Bioinformatics and Biomedical Technology |
| Pages | 329-331 |
| Number of pages | 3 |
| DOIs | |
| Publication status | Published - 2010 |
| Event | 2010 International Conference on Bioinformatics and Biomedical Technology, ICBBT 2010 - Chengdu, China Duration: 16 Apr 2010 → 18 Apr 2010 |
Publication series
| Name | ICBBT 2010 - 2010 International Conference on Bioinformatics and Biomedical Technology |
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Conference
| Conference | 2010 International Conference on Bioinformatics and Biomedical Technology, ICBBT 2010 |
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| Country/Territory | China |
| City | Chengdu |
| Period | 16/04/10 → 18/04/10 |
Keywords
- Activity
- Detection
- Solublecatechol-O-methyltransferase
- Temperature
- pH
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Yang, L., & Deng, Y. (2010). Study on activity of soluble catechol-O-methyltransferase in rat liver using high performance liquid chromatography. In ICBBT 2010 - 2010 International Conference on Bioinformatics and Biomedical Technology (pp. 329-331). Article 5478949 (ICBBT 2010 - 2010 International Conference on Bioinformatics and Biomedical Technology). https://doi.org/10.1109/ICBBT.2010.5478949