SPR Signal Amplification Based on Dynamic Field Enhancement at the Sensor Surface

Zhe Zhang; Rumeng Yi; Shanshan He; Lin Liu; Xiangdong Guo; Ran Gao; Zhi Mei Qi

doi:10.1109/JSEN.2021.3052556

SPR Signal Amplification Based on Dynamic Field Enhancement at the Sensor Surface

Zhe Zhang^*, Rumeng Yi, Shanshan He, Lin Liu, Xiangdong Guo, Ran Gao, Zhi Mei Qi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A new method of SPR signal amplification based on dynamic field enhancement at the sensor surface to detect C-reactive protein (CRP) is proposed. The gold nanoparticles-detection antibody (AuNPs-dAb) conjugates, as the dynamical agent, were applied to enhance the biosensor sensitivity. The mechanism of SPR sensitivity enhancement is due to the strong evanescent field coupling between the localized SPR (LSPR) of AuNPs and SPR of gold-silver alloy film which proved by COMSOL Multiphysics and MATLAB simulation. The biosensor sensitivity got with sandwich immunoassay (cAb/CRP/AuNPs-dAb) is 5.56-fold of the structure without AuNPs (cAb/CRP/dAb). Moreover, concentration of AuAg-SPR biosensor in CRP detection was lower for an order of magnitude than that of the conventional gold film cladding SPR (Au-SPR) biosensor, according to its direct reaction with the immobilized cAb. The dynamic field enhancement based AuAg-SPR biosensors have high sensitivity in detecting trace biomarker of CRP, and the improvement can also be applied to the detection of other biomarkers in low concentration sensitively.

Original language	English
Article number	9328342
Pages (from-to)	9523-9529
Number of pages	7
Journal	IEEE Sensors Journal
Volume	21
Issue number	7
DOIs	https://doi.org/10.1109/JSEN.2021.3052556
Publication status	Published - 1 Apr 2021
Externally published	Yes

Keywords

Surface plasmon resonance
dynamic field enhancement
gold nanoparticles
gold-silver alloy film
localized surface plasmon resonance
sensitivity

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title = "SPR Signal Amplification Based on Dynamic Field Enhancement at the Sensor Surface",

abstract = "A new method of SPR signal amplification based on dynamic field enhancement at the sensor surface to detect C-reactive protein (CRP) is proposed. The gold nanoparticles-detection antibody (AuNPs-dAb) conjugates, as the dynamical agent, were applied to enhance the biosensor sensitivity. The mechanism of SPR sensitivity enhancement is due to the strong evanescent field coupling between the localized SPR (LSPR) of AuNPs and SPR of gold-silver alloy film which proved by COMSOL Multiphysics and MATLAB simulation. The biosensor sensitivity got with sandwich immunoassay (cAb/CRP/AuNPs-dAb) is 5.56-fold of the structure without AuNPs (cAb/CRP/dAb). Moreover, concentration of AuAg-SPR biosensor in CRP detection was lower for an order of magnitude than that of the conventional gold film cladding SPR (Au-SPR) biosensor, according to its direct reaction with the immobilized cAb. The dynamic field enhancement based AuAg-SPR biosensors have high sensitivity in detecting trace biomarker of CRP, and the improvement can also be applied to the detection of other biomarkers in low concentration sensitively.",

keywords = "Surface plasmon resonance, dynamic field enhancement, gold nanoparticles, gold-silver alloy film, localized surface plasmon resonance, sensitivity",

author = "Zhe Zhang and Rumeng Yi and Shanshan He and Lin Liu and Xiangdong Guo and Ran Gao and Qi, {Zhi Mei}",

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AU - Zhang, Zhe

AU - Yi, Rumeng

AU - He, Shanshan

AU - Liu, Lin

AU - Guo, Xiangdong

AU - Gao, Ran

AU - Qi, Zhi Mei

PY - 2021/4/1

Y1 - 2021/4/1

N2 - A new method of SPR signal amplification based on dynamic field enhancement at the sensor surface to detect C-reactive protein (CRP) is proposed. The gold nanoparticles-detection antibody (AuNPs-dAb) conjugates, as the dynamical agent, were applied to enhance the biosensor sensitivity. The mechanism of SPR sensitivity enhancement is due to the strong evanescent field coupling between the localized SPR (LSPR) of AuNPs and SPR of gold-silver alloy film which proved by COMSOL Multiphysics and MATLAB simulation. The biosensor sensitivity got with sandwich immunoassay (cAb/CRP/AuNPs-dAb) is 5.56-fold of the structure without AuNPs (cAb/CRP/dAb). Moreover, concentration of AuAg-SPR biosensor in CRP detection was lower for an order of magnitude than that of the conventional gold film cladding SPR (Au-SPR) biosensor, according to its direct reaction with the immobilized cAb. The dynamic field enhancement based AuAg-SPR biosensors have high sensitivity in detecting trace biomarker of CRP, and the improvement can also be applied to the detection of other biomarkers in low concentration sensitively.

AB - A new method of SPR signal amplification based on dynamic field enhancement at the sensor surface to detect C-reactive protein (CRP) is proposed. The gold nanoparticles-detection antibody (AuNPs-dAb) conjugates, as the dynamical agent, were applied to enhance the biosensor sensitivity. The mechanism of SPR sensitivity enhancement is due to the strong evanescent field coupling between the localized SPR (LSPR) of AuNPs and SPR of gold-silver alloy film which proved by COMSOL Multiphysics and MATLAB simulation. The biosensor sensitivity got with sandwich immunoassay (cAb/CRP/AuNPs-dAb) is 5.56-fold of the structure without AuNPs (cAb/CRP/dAb). Moreover, concentration of AuAg-SPR biosensor in CRP detection was lower for an order of magnitude than that of the conventional gold film cladding SPR (Au-SPR) biosensor, according to its direct reaction with the immobilized cAb. The dynamic field enhancement based AuAg-SPR biosensors have high sensitivity in detecting trace biomarker of CRP, and the improvement can also be applied to the detection of other biomarkers in low concentration sensitively.

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KW - dynamic field enhancement

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KW - localized surface plasmon resonance

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ER -

SPR Signal Amplification Based on Dynamic Field Enhancement at the Sensor Surface

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