TY - JOUR
T1 - Single-shot Fourier ptychographic microscopy with isotropic lateral resolution via polarization-multiplexed LED illumination
AU - Zheng, Chuanjian
AU - Zhang, Shaohui
AU - Liu, Siying
AU - Yang, Delong
AU - Hao, Qun
N1 - Publisher Copyright:
© 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
PY - 2024/2/10
Y1 - 2024/2/10
N2 - Fourier ptychographic microscopy (FPM) has emerged as a new wide-field and high-resolution computational imaging technique in recent years. To ensure data redundancy for a stable convergence solution, conventional FPM requires dozens or hundreds of raw images, increasing the time cost for both data collection and computation. Here, we propose a single-shot Fourier ptychographic microscopy with isotropic lateral resolution via polarization-multiplexed LED illumination, termed SIFPM. Three LED elements covered with 0°/45°/135° polarization films, respectively, are used to provide numerical aperture-matched illumination for the sample simultaneously. Meanwhile, a polarization camera is utilized to record the light field distribution transmitted through the sample. Based on weak object transfer functions, we first obtain the amplitude and phase estimations of the sample by deconvolution, and then we use them as the initial guesses of the FPM algorithm to refine the accuracy of reconstruction. We validate the complex sample imaging performance of the proposed method on quantitative phase target, unstained and stained bio-samples. These results show that SIFPM can realize quantitative imaging for general samples with the resolution of the incoherent diffraction limit, permitting high-speed quantitative characterization for cells and tissues.
AB - Fourier ptychographic microscopy (FPM) has emerged as a new wide-field and high-resolution computational imaging technique in recent years. To ensure data redundancy for a stable convergence solution, conventional FPM requires dozens or hundreds of raw images, increasing the time cost for both data collection and computation. Here, we propose a single-shot Fourier ptychographic microscopy with isotropic lateral resolution via polarization-multiplexed LED illumination, termed SIFPM. Three LED elements covered with 0°/45°/135° polarization films, respectively, are used to provide numerical aperture-matched illumination for the sample simultaneously. Meanwhile, a polarization camera is utilized to record the light field distribution transmitted through the sample. Based on weak object transfer functions, we first obtain the amplitude and phase estimations of the sample by deconvolution, and then we use them as the initial guesses of the FPM algorithm to refine the accuracy of reconstruction. We validate the complex sample imaging performance of the proposed method on quantitative phase target, unstained and stained bio-samples. These results show that SIFPM can realize quantitative imaging for general samples with the resolution of the incoherent diffraction limit, permitting high-speed quantitative characterization for cells and tissues.
UR - http://www.scopus.com/inward/record.url?scp=85184152160&partnerID=8YFLogxK
U2 - 10.1364/BOE.513684
DO - 10.1364/BOE.513684
M3 - Article
AN - SCOPUS:85184152160
SN - 2156-7085
VL - 15
SP - 672
EP - 673
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 2
ER -