Self-controlled cell selection and loading system for & microfluidic systems

Huseyin Uvet*, Akiyuki Hasegawa, Kenichi Ohara, Tomohito Takubo, Yasushi Mae, Tatsuo Arai

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this paper, we presented a design for an automated cell supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel and then transport them to other modules. The system consists of two main parts; a single cell suction module, and a PDMS-based microfluidic chip controlled by an external pump. The desired number of vacuumed cells can be directed into the microfluidic chip and stored in a docking area. From the batch, they can be moved to next module by activating pneumatic pressure valves located on two sides of the chip.

Original languageEnglish
Title of host publication2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009
Pages4712-4717
Number of pages6
DOIs
Publication statusPublished - 11 Dec 2009
Externally publishedYes
Event2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009 - St. Louis, MO, United States
Duration: 11 Oct 200915 Oct 2009

Publication series

Name2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009

Conference

Conference2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009
Country/TerritoryUnited States
CitySt. Louis, MO
Period11/10/0915/10/09

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Uvet, H., Hasegawa, A., Ohara, K., Takubo, T., Mae, Y., & Arai, T. (2009). Self-controlled cell selection and loading system for & microfluidic systems. In 2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009 (pp. 4712-4717). Article 5354585 (2009 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2009). https://doi.org/10.1109/IROS.2009.5354585