Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

Juan Song; Han Zhang Mou; Xiao Qiong Li; Yu Liu; Xue Jiao Yang; Hong Yuan Chen; Jing Juan Xu

doi:10.1016/j.snb.2022.131644

Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

Juan Song, Han Zhang Mou, Xiao Qiong Li, Yu Liu, Xue Jiao Yang^*, Hong Yuan Chen, Jing Juan Xu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

In situ visual detection of microRNAs (miRNAs) is of great significance for early diagnosis of miRNA-related diseases. In this study, we designed a rolling circle replication-driven self-assembled nucleic acid nanosphere (NS) for imaging miRNAs in living cells, which was loaded with four functional oligonucleotides (THp, H1, H2, H3) through hybridization. The DNA/RNA NS showed excellent biocompatibility, stability and RNase H-responsiveness, enabling on-demand delivery of sensing components and live-cell applications. In the presence of the target miRNA, the cascade amplification of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) would be triggered to generate a strong fluorescence signal. Taking miRNA 155 as a model, the developed DNA/RNA NS achieved sensitive detection of the target in vitro and in tumor cells, demonstrating the potential of miRNA-based tumor screening.

Original language	English
Article number	131644
Journal	Sensors and Actuators, B: Chemical
Volume	360
DOIs	https://doi.org/10.1016/j.snb.2022.131644
Publication status	Published - 1 Jun 2022
Externally published	Yes

Keywords

Cascade amplification
CHA and HCR
DNA/RNA nanosphere
MiRNA 155
On-demand delivery

Access to Document

10.1016/j.snb.2022.131644

Cite this

Song, J., Mou, H. Z., Li, X. Q., Liu, Y., Yang, X. J., Chen, H. Y., & Xu, J. J. (2022). Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging. Sensors and Actuators, B: Chemical, 360, Article 131644. https://doi.org/10.1016/j.snb.2022.131644

@article{52aa5371a30247aeb9b6842d6cbc22ef,

title = "Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging",

abstract = "In situ visual detection of microRNAs (miRNAs) is of great significance for early diagnosis of miRNA-related diseases. In this study, we designed a rolling circle replication-driven self-assembled nucleic acid nanosphere (NS) for imaging miRNAs in living cells, which was loaded with four functional oligonucleotides (THp, H1, H2, H3) through hybridization. The DNA/RNA NS showed excellent biocompatibility, stability and RNase H-responsiveness, enabling on-demand delivery of sensing components and live-cell applications. In the presence of the target miRNA, the cascade amplification of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) would be triggered to generate a strong fluorescence signal. Taking miRNA 155 as a model, the developed DNA/RNA NS achieved sensitive detection of the target in vitro and in tumor cells, demonstrating the potential of miRNA-based tumor screening.",

keywords = "Cascade amplification, CHA and HCR, DNA/RNA nanosphere, MiRNA 155, On-demand delivery",

author = "Juan Song and Mou, {Han Zhang} and Li, {Xiao Qiong} and Yu Liu and Yang, {Xue Jiao} and Chen, {Hong Yuan} and Xu, {Jing Juan}",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = jun,

day = "1",

doi = "10.1016/j.snb.2022.131644",

language = "English",

volume = "360",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

AU - Song, Juan

AU - Mou, Han Zhang

AU - Li, Xiao Qiong

AU - Liu, Yu

AU - Yang, Xue Jiao

AU - Chen, Hong Yuan

AU - Xu, Jing Juan

PY - 2022/6/1

Y1 - 2022/6/1

N2 - In situ visual detection of microRNAs (miRNAs) is of great significance for early diagnosis of miRNA-related diseases. In this study, we designed a rolling circle replication-driven self-assembled nucleic acid nanosphere (NS) for imaging miRNAs in living cells, which was loaded with four functional oligonucleotides (THp, H1, H2, H3) through hybridization. The DNA/RNA NS showed excellent biocompatibility, stability and RNase H-responsiveness, enabling on-demand delivery of sensing components and live-cell applications. In the presence of the target miRNA, the cascade amplification of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) would be triggered to generate a strong fluorescence signal. Taking miRNA 155 as a model, the developed DNA/RNA NS achieved sensitive detection of the target in vitro and in tumor cells, demonstrating the potential of miRNA-based tumor screening.

AB - In situ visual detection of microRNAs (miRNAs) is of great significance for early diagnosis of miRNA-related diseases. In this study, we designed a rolling circle replication-driven self-assembled nucleic acid nanosphere (NS) for imaging miRNAs in living cells, which was loaded with four functional oligonucleotides (THp, H1, H2, H3) through hybridization. The DNA/RNA NS showed excellent biocompatibility, stability and RNase H-responsiveness, enabling on-demand delivery of sensing components and live-cell applications. In the presence of the target miRNA, the cascade amplification of catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) would be triggered to generate a strong fluorescence signal. Taking miRNA 155 as a model, the developed DNA/RNA NS achieved sensitive detection of the target in vitro and in tumor cells, demonstrating the potential of miRNA-based tumor screening.

KW - Cascade amplification

KW - CHA and HCR

KW - DNA/RNA nanosphere

KW - MiRNA 155

KW - On-demand delivery

UR - http://www.scopus.com/inward/record.url?scp=85126117346&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2022.131644

DO - 10.1016/j.snb.2022.131644

M3 - Article

AN - SCOPUS:85126117346

SN - 0925-4005

VL - 360

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

M1 - 131644

ER -

Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this