Scanning Electrochemical Photometric Sensors for Label-Free Single-Cell Imaging and Quantitative Absorption Analysis

Jian Wang; Yulan Tian; Fangming Chen; Wei Chen; Liping Du; Zhiyuan He; Chunsheng Wu; De Wen Zhang

doi:10.1021/acs.analchem.0c01118

Scanning Electrochemical Photometric Sensors for Label-Free Single-Cell Imaging and Quantitative Absorption Analysis

Jian Wang, Yulan Tian, Fangming Chen, Wei Chen, Liping Du, Zhiyuan He, Chunsheng Wu, De Wen Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A new photoelectrochemical imaging method termed scanning electrochemical photometric sensor (SEPS) is proposed in this work. It was derived from light-addressable potentiometric sensor (LAPS) and scanning photoinduced impedance microscopy (SPIM) using a front-side laser illumination at a field-effect structure. When the laser beam scans across the sensor substrate, local photocurrent changes at inversion due to the light absorption of analytes can be recorded. It will be shown that SEPS could be used for label-free living cell imaging with micro-resolution as well as real-time quantitative absorption analysis, which would broaden the applications of traditional LAPS/SPIM from potentiometric/impedance measurements to local optical analysis.

Original language	English
Pages (from-to)	9739-9744
Number of pages	6
Journal	Analytical Chemistry
Volume	92
Issue number	14
DOIs	https://doi.org/10.1021/acs.analchem.0c01118
Publication status	Published - 21 Jul 2020
Externally published	Yes

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title = "Scanning Electrochemical Photometric Sensors for Label-Free Single-Cell Imaging and Quantitative Absorption Analysis",

abstract = "A new photoelectrochemical imaging method termed scanning electrochemical photometric sensor (SEPS) is proposed in this work. It was derived from light-addressable potentiometric sensor (LAPS) and scanning photoinduced impedance microscopy (SPIM) using a front-side laser illumination at a field-effect structure. When the laser beam scans across the sensor substrate, local photocurrent changes at inversion due to the light absorption of analytes can be recorded. It will be shown that SEPS could be used for label-free living cell imaging with micro-resolution as well as real-time quantitative absorption analysis, which would broaden the applications of traditional LAPS/SPIM from potentiometric/impedance measurements to local optical analysis.",

author = "Jian Wang and Yulan Tian and Fangming Chen and Wei Chen and Liping Du and Zhiyuan He and Chunsheng Wu and Zhang, {De Wen}",

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T1 - Scanning Electrochemical Photometric Sensors for Label-Free Single-Cell Imaging and Quantitative Absorption Analysis

AU - Wang, Jian

AU - Tian, Yulan

AU - Chen, Fangming

AU - Chen, Wei

AU - Du, Liping

AU - He, Zhiyuan

AU - Wu, Chunsheng

AU - Zhang, De Wen

PY - 2020/7/21

Y1 - 2020/7/21

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AB - A new photoelectrochemical imaging method termed scanning electrochemical photometric sensor (SEPS) is proposed in this work. It was derived from light-addressable potentiometric sensor (LAPS) and scanning photoinduced impedance microscopy (SPIM) using a front-side laser illumination at a field-effect structure. When the laser beam scans across the sensor substrate, local photocurrent changes at inversion due to the light absorption of analytes can be recorded. It will be shown that SEPS could be used for label-free living cell imaging with micro-resolution as well as real-time quantitative absorption analysis, which would broaden the applications of traditional LAPS/SPIM from potentiometric/impedance measurements to local optical analysis.

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JO - Analytical Chemistry

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Scanning Electrochemical Photometric Sensors for Label-Free Single-Cell Imaging and Quantitative Absorption Analysis

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