TY - GEN
T1 - Salsolinol, a dopamine-derived tetrahydroisoquinoline, occur in dopaminergic SH-SY5Y cells induced by dopamine
AU - Wang, Ran
AU - Yang, Wei
AU - Luan, Yujing
AU - Mao, Jian
AU - Qing, Hong
AU - Deng, Yulin
PY - 2007
Y1 - 2007
N2 - The dopamine-derived salsolinol (Sal), 1-methyl-6,7-dihydroxy-1,2,3,4- tetrahydroisoquinoline, has been considered a potential endogenous neurotoxin involved in the etiology of PD. In the present study, 150μM dopamine (DA) was applied to be the treatment concentration on account of that 15OuJVI DA decreased cell viability to 44.8%. The level of salsolinol in human dopaminergic SH-SY5Y cells treated with 150uM DA for 24h was measured using high-performance liquid chromatography with electrochemical detection (HPLC-ECD), and the possible mechanism of salsolinol formation in SH-SY5Y cells was discussed. DA significantly increased the production of hydroxyl radicals quantitatively measured after derivatization as 2, 3- and 2, 5-DHBA by reaction with salicylic acid, and malonaldehyde (MDA), a marker of lipid peroxidation. Salsolinol increased remarkably in DA-induced cells compared to control cells. These results suggest that DA incubation and oxidative stress induced by excessive dopamine contribute to the synthesis of salsolinol in SH-SH5Y cells.
AB - The dopamine-derived salsolinol (Sal), 1-methyl-6,7-dihydroxy-1,2,3,4- tetrahydroisoquinoline, has been considered a potential endogenous neurotoxin involved in the etiology of PD. In the present study, 150μM dopamine (DA) was applied to be the treatment concentration on account of that 15OuJVI DA decreased cell viability to 44.8%. The level of salsolinol in human dopaminergic SH-SY5Y cells treated with 150uM DA for 24h was measured using high-performance liquid chromatography with electrochemical detection (HPLC-ECD), and the possible mechanism of salsolinol formation in SH-SY5Y cells was discussed. DA significantly increased the production of hydroxyl radicals quantitatively measured after derivatization as 2, 3- and 2, 5-DHBA by reaction with salicylic acid, and malonaldehyde (MDA), a marker of lipid peroxidation. Salsolinol increased remarkably in DA-induced cells compared to control cells. These results suggest that DA incubation and oxidative stress induced by excessive dopamine contribute to the synthesis of salsolinol in SH-SH5Y cells.
UR - http://www.scopus.com/inward/record.url?scp=48149087221&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2007.4381975
DO - 10.1109/ICCME.2007.4381975
M3 - Conference contribution
AN - SCOPUS:48149087221
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1404
EP - 1408
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -
