Replacing 99mTc with 111In improves MORF/cMORF pretargeting by reducing intestinal accumulation

Guozheng Liu; Dengfeng Cheng; Shuping Dou; Xiangji Chen; Minmin Liang; P. Hendrik Pretorius; Mary Rusckowski; Donald J. Hnatowich

doi:10.1007/s11307-009-0209-0

Replacing ^99mTc with ¹¹¹In improves MORF/cMORF pretargeting by reducing intestinal accumulation

Guozheng Liu^*, Dengfeng Cheng, Shuping Dou, Xiangji Chen, Minmin Liang, P. Hendrik Pretorius, Mary Rusckowski, Donald J. Hnatowich

^*Corresponding author for this work

University of Massachusetts Medical School

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Purpose: To reduce accumulation in the abdomen by MORF/cMORF pretargeting, ¹¹¹In was compared to ^99mTc as the radiolabel. Procedures: After receiving either ^99mTc (MAG3)-cMORF or ¹¹¹In (DTPA)-cMORF, normal mice were imaged and killed for pharmacokinetics. Thereafter, tumored mice were pretargeted with MORF-antibody, 48 h later were given an injection of ^99mTc- or ¹¹¹In-cMORF, and finally were imaged repeatedly. Results: The cMORF biodistribution in both normal and pretargeted tumored mice was influenced by its radiolabel. While excretion of both ^99mTc-cMORF and ¹¹¹In-cMORF was rapid and mainly through the kidneys, about 2% of ^99mTc accumulated in the intestines compared to essentially no intestinal accumulation for ¹¹¹In at any time. Tumor accumulation was unchanged. Conclusion: In applications of MORF/cMORF pretargeting intended to image organs deep within the abdomen such as the pancreas, radiolabeling with ¹¹¹In may be superior to ^99mTc.

Original language	English
Pages (from-to)	303-307
Number of pages	5
Journal	Molecular Imaging and Biology
Volume	11
Issue number	5
DOIs	https://doi.org/10.1007/s11307-009-0209-0
Publication status	Published - 2009
Externally published	Yes

Keywords

Antibodies
Anticancer
Pretargeting
Radioimmunotargeting
Tumor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s11307-009-0209-0

Cite this

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title = "Replacing 99mTc with 111In improves MORF/cMORF pretargeting by reducing intestinal accumulation",

abstract = "Purpose: To reduce accumulation in the abdomen by MORF/cMORF pretargeting, 111In was compared to 99mTc as the radiolabel. Procedures: After receiving either 99mTc (MAG3)-cMORF or 111In (DTPA)-cMORF, normal mice were imaged and killed for pharmacokinetics. Thereafter, tumored mice were pretargeted with MORF-antibody, 48 h later were given an injection of 99mTc- or 111In-cMORF, and finally were imaged repeatedly. Results: The cMORF biodistribution in both normal and pretargeted tumored mice was influenced by its radiolabel. While excretion of both 99mTc-cMORF and 111In-cMORF was rapid and mainly through the kidneys, about 2% of 99mTc accumulated in the intestines compared to essentially no intestinal accumulation for 111In at any time. Tumor accumulation was unchanged. Conclusion: In applications of MORF/cMORF pretargeting intended to image organs deep within the abdomen such as the pancreas, radiolabeling with 111In may be superior to 99mTc.",

keywords = "Antibodies, Anticancer, Pretargeting, Radioimmunotargeting, Tumor",

author = "Guozheng Liu and Dengfeng Cheng and Shuping Dou and Xiangji Chen and Minmin Liang and Pretorius, {P. Hendrik} and Mary Rusckowski and Hnatowich, {Donald J.}",

year = "2009",

doi = "10.1007/s11307-009-0209-0",

language = "English",

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pages = "303--307",

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T1 - Replacing 99mTc with 111In improves MORF/cMORF pretargeting by reducing intestinal accumulation

AU - Liu, Guozheng

AU - Cheng, Dengfeng

AU - Dou, Shuping

AU - Chen, Xiangji

AU - Liang, Minmin

AU - Pretorius, P. Hendrik

AU - Rusckowski, Mary

AU - Hnatowich, Donald J.

PY - 2009

Y1 - 2009

N2 - Purpose: To reduce accumulation in the abdomen by MORF/cMORF pretargeting, 111In was compared to 99mTc as the radiolabel. Procedures: After receiving either 99mTc (MAG3)-cMORF or 111In (DTPA)-cMORF, normal mice were imaged and killed for pharmacokinetics. Thereafter, tumored mice were pretargeted with MORF-antibody, 48 h later were given an injection of 99mTc- or 111In-cMORF, and finally were imaged repeatedly. Results: The cMORF biodistribution in both normal and pretargeted tumored mice was influenced by its radiolabel. While excretion of both 99mTc-cMORF and 111In-cMORF was rapid and mainly through the kidneys, about 2% of 99mTc accumulated in the intestines compared to essentially no intestinal accumulation for 111In at any time. Tumor accumulation was unchanged. Conclusion: In applications of MORF/cMORF pretargeting intended to image organs deep within the abdomen such as the pancreas, radiolabeling with 111In may be superior to 99mTc.

AB - Purpose: To reduce accumulation in the abdomen by MORF/cMORF pretargeting, 111In was compared to 99mTc as the radiolabel. Procedures: After receiving either 99mTc (MAG3)-cMORF or 111In (DTPA)-cMORF, normal mice were imaged and killed for pharmacokinetics. Thereafter, tumored mice were pretargeted with MORF-antibody, 48 h later were given an injection of 99mTc- or 111In-cMORF, and finally were imaged repeatedly. Results: The cMORF biodistribution in both normal and pretargeted tumored mice was influenced by its radiolabel. While excretion of both 99mTc-cMORF and 111In-cMORF was rapid and mainly through the kidneys, about 2% of 99mTc accumulated in the intestines compared to essentially no intestinal accumulation for 111In at any time. Tumor accumulation was unchanged. Conclusion: In applications of MORF/cMORF pretargeting intended to image organs deep within the abdomen such as the pancreas, radiolabeling with 111In may be superior to 99mTc.

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