Reduction of 4-nitrophenol catalyzed by nitroreductase

Jie Chen; Rong Ji Dai; Bin Tong; Sheng Yuan Xiao; Weiwei Meng

doi:10.1016/j.cclet.2006.11.009

Reduction of 4-nitrophenol catalyzed by nitroreductase

Jie Chen, Rong Ji Dai^*, Bin Tong, Sheng Yuan Xiao, Weiwei Meng

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.

Original language	English
Pages (from-to)	10-12
Number of pages	3
Journal	Chinese Chemical Letters
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/j.cclet.2006.11.009
Publication status	Published - Jan 2007

Keywords

4-Nitrophenol
Biotransformation
Nitroreductase
Reduction

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10.1016/j.cclet.2006.11.009

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title = "Reduction of 4-nitrophenol catalyzed by nitroreductase",

abstract = "The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.",

keywords = "4-Nitrophenol, Biotransformation, Nitroreductase, Reduction",

author = "Jie Chen and Dai, {Rong Ji} and Bin Tong and Xiao, {Sheng Yuan} and Weiwei Meng",

year = "2007",

month = jan,

doi = "10.1016/j.cclet.2006.11.009",

language = "English",

volume = "18",

pages = "10--12",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

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TY - JOUR

T1 - Reduction of 4-nitrophenol catalyzed by nitroreductase

AU - Chen, Jie

AU - Dai, Rong Ji

AU - Tong, Bin

AU - Xiao, Sheng Yuan

AU - Meng, Weiwei

PY - 2007/1

Y1 - 2007/1

N2 - The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.

AB - The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.

KW - 4-Nitrophenol

KW - Biotransformation

KW - Nitroreductase

KW - Reduction

UR - http://www.scopus.com/inward/record.url?scp=33847142185&partnerID=8YFLogxK

U2 - 10.1016/j.cclet.2006.11.009

DO - 10.1016/j.cclet.2006.11.009

M3 - Article

AN - SCOPUS:33847142185

SN - 1001-8417

VL - 18

SP - 10

EP - 12

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 1

ER -

Reduction of 4-nitrophenol catalyzed by nitroreductase

Abstract

Keywords

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