Abstract
The method of reversed-phase high performance liquid chromatography was established for determination of effective components in OC-CS spray using double wavelength UV detection under following conditions: a column of Kromasil C18 (250 mm×4.6 mm, 5 μm), mobile phase composed of methanol/water (80/20) at a flow rate of 0.8 mL/min, column temperature of 25°C; and the UV detection at 227 nm and 300 nm. Three key components in OC-CS spray were distinguished clearly, i.e. o-chlorobenzalmalononitrile (CS), oleoresin capsicum (OC) and dihydrocapsaicin (DC). This method is fast and simple, with satisfactory linear relationship between UV absorption and concentration. It is instructive for modifying the standard method for detection of related components in the spray.
| Original language | English |
|---|---|
| Pages (from-to) | 1035-1038 |
| Number of pages | 4 |
| Journal | Binggong Xuebao/Acta Armamentarii |
| Volume | 30 |
| Issue number | 8 |
| Publication status | Published - Aug 2009 |
Keywords
- Analytical chemistry
- Dihydrocapsaicin
- High performance liquid chromatography
- O-chlorobenzalmalononitrile
- OC-CS sprays
- Oleoresin capsicum