Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation

Heng Zhang; Da Xing; Shengnan Wu; Xuegang Sun

doi:10.1117/12.841566

Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation

Heng Zhang, Da Xing^*, Shengnan Wu, Xuegang Sun

^*Corresponding author for this work

South China Normal University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm² after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.

Original language	English
Title of host publication	8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
DOIs	https://doi.org/10.1117/12.841566
Publication status	Published - 2009
Externally published	Yes
Event	8th International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2009 - Wuhan, China Duration: 8 Aug 2009 → 10 Aug 2009

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7519
ISSN (Print)	0277-786X

Conference

Conference	8th International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2009
Country/Territory	China
City	Wuhan
Period	8/08/09 → 10/08/09

Keywords

Apoptosis
HF-LPLI
PKCδ

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10.1117/12.841566

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Zhang, H., Xing, D., Wu, S., & Sun, X. (2009). Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation. In 8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009) Article 751919 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7519). https://doi.org/10.1117/12.841566

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title = "Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation",

abstract = "Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm2 after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.",

keywords = "Apoptosis, HF-LPLI, PKCδ",

author = "Heng Zhang and Da Xing and Shengnan Wu and Xuegang Sun",

year = "2009",

doi = "10.1117/12.841566",

language = "English",

isbn = "9780819479082",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)",

note = "8th International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2009 ; Conference date: 08-08-2009 Through 10-08-2009",

}

Zhang, H, Xing, D, Wu, S & Sun, X 2009, Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation. in 8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)., 751919, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7519, 8th International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2009, Wuhan, China, 8/08/09. https://doi.org/10.1117/12.841566

Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation. / Zhang, Heng; Xing, Da; Wu, Shengnan et al.
8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009). 2009. 751919 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7519).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation

AU - Zhang, Heng

AU - Xing, Da

AU - Wu, Shengnan

AU - Sun, Xuegang

PY - 2009

Y1 - 2009

N2 - Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm2 after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.

AB - Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm2 after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.

KW - Apoptosis

KW - HF-LPLI

KW - PKCδ

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U2 - 10.1117/12.841566

DO - 10.1117/12.841566

M3 - Conference contribution

AN - SCOPUS:71549149996

SN - 9780819479082

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - 8th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)

T2 - 8th International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2009

Y2 - 8 August 2009 through 10 August 2009

ER -

Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation

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