Probing the fractal pattern of heartbeats in drosophila pupae by visible optical recording system

Chen Lin; Yi Chung Chang; Ya Chen Cheng; Po Jung Lai; Chien Hung Yeh; Wan Hsin Hsieh; Kun Hu; June Tai Wu; Hsiu Hsiang Lee; Men Tzung Lo; Yi Lwun Ho

doi:10.1038/srep31950

Probing the fractal pattern of heartbeats in drosophila pupae by visible optical recording system

Chen Lin, Yi Chung Chang, Ya Chen Cheng, Po Jung Lai, Chien Hung Yeh, Wan Hsin Hsieh, Kun Hu, June Tai Wu, Hsiu Hsiang Lee, Men Tzung Lo^*, Yi Lwun Ho

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Judiciously tuning heart rates is critical for regular cardiovascular function. The fractal pattern of heartbeats-a multiscale regulation in instantaneous fluctuations-is well known for vertebrates. The most primitive heart system of the Drosophila provides a useful model to understand the evolutional origin of such a fractal pattern as well as the alterations of fractal pattern during diseased statuses. We developed a non-invasive visible optical heart rate recording system especially suitable for long-term recording by using principal component analysis (PCA) instead of fluorescence recording system to avoid the confounding effect from intense light irradiation. To deplete intracellular Ca 2+ levels, the expression of sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCA) was tissue-specifically knocked down. The SERCA group shows longer heart beat intervals (Mean ± SD: 1009.7 ± 151.6 ms) as compared to the control group (545.5 ± 45.4 ms, p < 0.001). The multiscale correlation of SERCA group (scaling exponent: 0.77 ± 0.07), on the other hand, is weaker than that of the control Drosophila (scaling exponent: 0.85 ± 0.03) (p = 0.016).

Original language	English
Article number	31950
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep31950
Publication status	Published - 18 Aug 2016
Externally published	Yes

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title = "Probing the fractal pattern of heartbeats in drosophila pupae by visible optical recording system",

abstract = "Judiciously tuning heart rates is critical for regular cardiovascular function. The fractal pattern of heartbeats-a multiscale regulation in instantaneous fluctuations-is well known for vertebrates. The most primitive heart system of the Drosophila provides a useful model to understand the evolutional origin of such a fractal pattern as well as the alterations of fractal pattern during diseased statuses. We developed a non-invasive visible optical heart rate recording system especially suitable for long-term recording by using principal component analysis (PCA) instead of fluorescence recording system to avoid the confounding effect from intense light irradiation. To deplete intracellular Ca 2+ levels, the expression of sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCA) was tissue-specifically knocked down. The SERCA group shows longer heart beat intervals (Mean ± SD: 1009.7 ± 151.6 ms) as compared to the control group (545.5 ± 45.4 ms, p < 0.001). The multiscale correlation of SERCA group (scaling exponent: 0.77 ± 0.07), on the other hand, is weaker than that of the control Drosophila (scaling exponent: 0.85 ± 0.03) (p = 0.016).",

author = "Chen Lin and Chang, {Yi Chung} and Cheng, {Ya Chen} and Lai, {Po Jung} and Yeh, {Chien Hung} and Hsieh, {Wan Hsin} and Kun Hu and Wu, {June Tai} and Lee, {Hsiu Hsiang} and Lo, {Men Tzung} and Ho, {Yi Lwun}",

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year = "2016",

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doi = "10.1038/srep31950",

language = "English",

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journal = "Scientific Reports",

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T1 - Probing the fractal pattern of heartbeats in drosophila pupae by visible optical recording system

AU - Lin, Chen

AU - Chang, Yi Chung

AU - Cheng, Ya Chen

AU - Lai, Po Jung

AU - Yeh, Chien Hung

AU - Hsieh, Wan Hsin

AU - Hu, Kun

AU - Wu, June Tai

AU - Lee, Hsiu Hsiang

AU - Lo, Men Tzung

AU - Ho, Yi Lwun

PY - 2016/8/18

Y1 - 2016/8/18

N2 - Judiciously tuning heart rates is critical for regular cardiovascular function. The fractal pattern of heartbeats-a multiscale regulation in instantaneous fluctuations-is well known for vertebrates. The most primitive heart system of the Drosophila provides a useful model to understand the evolutional origin of such a fractal pattern as well as the alterations of fractal pattern during diseased statuses. We developed a non-invasive visible optical heart rate recording system especially suitable for long-term recording by using principal component analysis (PCA) instead of fluorescence recording system to avoid the confounding effect from intense light irradiation. To deplete intracellular Ca 2+ levels, the expression of sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCA) was tissue-specifically knocked down. The SERCA group shows longer heart beat intervals (Mean ± SD: 1009.7 ± 151.6 ms) as compared to the control group (545.5 ± 45.4 ms, p < 0.001). The multiscale correlation of SERCA group (scaling exponent: 0.77 ± 0.07), on the other hand, is weaker than that of the control Drosophila (scaling exponent: 0.85 ± 0.03) (p = 0.016).

AB - Judiciously tuning heart rates is critical for regular cardiovascular function. The fractal pattern of heartbeats-a multiscale regulation in instantaneous fluctuations-is well known for vertebrates. The most primitive heart system of the Drosophila provides a useful model to understand the evolutional origin of such a fractal pattern as well as the alterations of fractal pattern during diseased statuses. We developed a non-invasive visible optical heart rate recording system especially suitable for long-term recording by using principal component analysis (PCA) instead of fluorescence recording system to avoid the confounding effect from intense light irradiation. To deplete intracellular Ca 2+ levels, the expression of sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCA) was tissue-specifically knocked down. The SERCA group shows longer heart beat intervals (Mean ± SD: 1009.7 ± 151.6 ms) as compared to the control group (545.5 ± 45.4 ms, p < 0.001). The multiscale correlation of SERCA group (scaling exponent: 0.77 ± 0.07), on the other hand, is weaker than that of the control Drosophila (scaling exponent: 0.85 ± 0.03) (p = 0.016).

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Probing the fractal pattern of heartbeats in drosophila pupae by visible optical recording system

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