Preparation of thermo-response borinate affinity chromatography of low-pressure and low-flow

Yan Li Liang; Lin Lin Dong; Shan Shan Wang; Yu Shi; Rong Ji Dai; Wei Wei Meng; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.004

Preparation of thermo-response borinate affinity chromatography of low-pressure and low-flow

Yan Li Liang, Lin Lin Dong, Shan Shan Wang, Yu Shi, Rong Ji Dai^*, Wei Wei Meng, Yu Lin Deng

^*Corresponding author for this work

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Poly (N-isopropyl acrylamide-co-4-vinyl benzene boric acid) (P (NIPAAm-co-VPBA)) modified liquid chromatographic stationary phase on silica gel was prepared by atom transfer radical polymerization (ATRP) and was characterized by Fourier infrared spectrum, X-ray photoelectron spectroscopy. Thermogravimetric analysis was performed to determine its grafting ratio. The retention behavior of adenosine and deoxyadensine was studied by HPLC at low-pressure and low-flow velocity. The rate of recovery is 85.4%. The results indicate that the enrichment of adenosine can be realized in this self-made device, which can provide theoretical foundation and technical support for spatial device.

Original language	English
Pages (from-to)	16-19 and 44
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.004
Publication status	Published - 1 Jul 2015

Keywords

Adenosine
Boric acid
Spatial device
Thermo-response

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10.15918/j.tbit1001-0645.2015.supplement1.004

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title = "Preparation of thermo-response borinate affinity chromatography of low-pressure and low-flow",

abstract = "Poly (N-isopropyl acrylamide-co-4-vinyl benzene boric acid) (P (NIPAAm-co-VPBA)) modified liquid chromatographic stationary phase on silica gel was prepared by atom transfer radical polymerization (ATRP) and was characterized by Fourier infrared spectrum, X-ray photoelectron spectroscopy. Thermogravimetric analysis was performed to determine its grafting ratio. The retention behavior of adenosine and deoxyadensine was studied by HPLC at low-pressure and low-flow velocity. The rate of recovery is 85.4%. The results indicate that the enrichment of adenosine can be realized in this self-made device, which can provide theoretical foundation and technical support for spatial device.",

keywords = "Adenosine, Boric acid, Spatial device, Thermo-response",

author = "Liang, {Yan Li} and Dong, {Lin Lin} and Wang, {Shan Shan} and Yu Shi and Dai, {Rong Ji} and Meng, {Wei Wei} and Deng, {Yu Lin}",

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doi = "10.15918/j.tbit1001-0645.2015.supplement1.004",

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TY - JOUR

T1 - Preparation of thermo-response borinate affinity chromatography of low-pressure and low-flow

AU - Liang, Yan Li

AU - Dong, Lin Lin

AU - Wang, Shan Shan

AU - Shi, Yu

AU - Dai, Rong Ji

AU - Meng, Wei Wei

AU - Deng, Yu Lin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Poly (N-isopropyl acrylamide-co-4-vinyl benzene boric acid) (P (NIPAAm-co-VPBA)) modified liquid chromatographic stationary phase on silica gel was prepared by atom transfer radical polymerization (ATRP) and was characterized by Fourier infrared spectrum, X-ray photoelectron spectroscopy. Thermogravimetric analysis was performed to determine its grafting ratio. The retention behavior of adenosine and deoxyadensine was studied by HPLC at low-pressure and low-flow velocity. The rate of recovery is 85.4%. The results indicate that the enrichment of adenosine can be realized in this self-made device, which can provide theoretical foundation and technical support for spatial device.

AB - Poly (N-isopropyl acrylamide-co-4-vinyl benzene boric acid) (P (NIPAAm-co-VPBA)) modified liquid chromatographic stationary phase on silica gel was prepared by atom transfer radical polymerization (ATRP) and was characterized by Fourier infrared spectrum, X-ray photoelectron spectroscopy. Thermogravimetric analysis was performed to determine its grafting ratio. The retention behavior of adenosine and deoxyadensine was studied by HPLC at low-pressure and low-flow velocity. The rate of recovery is 85.4%. The results indicate that the enrichment of adenosine can be realized in this self-made device, which can provide theoretical foundation and technical support for spatial device.

KW - Adenosine

KW - Boric acid

KW - Spatial device

KW - Thermo-response

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U2 - 10.15918/j.tbit1001-0645.2015.supplement1.004

DO - 10.15918/j.tbit1001-0645.2015.supplement1.004

M3 - Article

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SN - 1001-0645

VL - 35

SP - 16-19 and 44

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Preparation of thermo-response borinate affinity chromatography of low-pressure and low-flow

Abstract

Keywords

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