Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids

Zong Jian Liu; Rong Ji Dai; Xiao Liang; Hai Yan Wu; Hao Gu; Wei Ding; Qing Qing Qian; Yu Lin Deng

Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids

Zong Jian Liu, Rong Ji Dai^*, Xiao Liang, Hai Yan Wu, Hao Gu, Wei Ding, Qing Qing Qian, Yu Lin Deng

^*Corresponding author for this work

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Chromatographic packing materials containing poly(N-isopropylacrylamide-co-2-acr-ylamido-2-methyl propane sulfonic acid) and poly(N-isopropylacrylamide-co-N-tert-butylacryla-mide) were prepared via conventional radical polymerization. Characterization was performed with thermogravimetric analysis (TGA) and elemental analysis to determine the grafting ratio. Mixtures of Val, Leu, Phe, Trp, Art and Ala derivatized with phenylisothiocyanate were separated using prepared stationary phases by HPLC. The results indicated that separation can be accomplished only by controlling column temperatures, which proves that chromatographic retention of analytes alters with the change of column temperature, and the materials are of temperature-responsive property.

Original language	English
Pages (from-to)	1104-1108
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	31
Issue number	9
Publication status	Published - Sept 2011

Keywords

Chromatographic separation
Poly(N-isopropylacrylamide)
Temperature-responsive polymer

Cite this

Liu, Z. J., Dai, R. J., Liang, X., Wu, H. Y., Gu, H., Ding, W., Qian, Q. Q., & Deng, Y. L. (2011). Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 31(9), 1104-1108.

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title = "Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids",

abstract = "Chromatographic packing materials containing poly(N-isopropylacrylamide-co-2-acr-ylamido-2-methyl propane sulfonic acid) and poly(N-isopropylacrylamide-co-N-tert-butylacryla-mide) were prepared via conventional radical polymerization. Characterization was performed with thermogravimetric analysis (TGA) and elemental analysis to determine the grafting ratio. Mixtures of Val, Leu, Phe, Trp, Art and Ala derivatized with phenylisothiocyanate were separated using prepared stationary phases by HPLC. The results indicated that separation can be accomplished only by controlling column temperatures, which proves that chromatographic retention of analytes alters with the change of column temperature, and the materials are of temperature-responsive property.",

keywords = "Chromatographic separation, Poly(N-isopropylacrylamide), Temperature-responsive polymer",

author = "Liu, {Zong Jian} and Dai, {Rong Ji} and Xiao Liang and Wu, {Hai Yan} and Hao Gu and Wei Ding and Qian, {Qing Qing} and Deng, {Yu Lin}",

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T1 - Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids

AU - Liu, Zong Jian

AU - Dai, Rong Ji

AU - Liang, Xiao

AU - Wu, Hai Yan

AU - Gu, Hao

AU - Ding, Wei

AU - Qian, Qing Qing

AU - Deng, Yu Lin

PY - 2011/9

Y1 - 2011/9

N2 - Chromatographic packing materials containing poly(N-isopropylacrylamide-co-2-acr-ylamido-2-methyl propane sulfonic acid) and poly(N-isopropylacrylamide-co-N-tert-butylacryla-mide) were prepared via conventional radical polymerization. Characterization was performed with thermogravimetric analysis (TGA) and elemental analysis to determine the grafting ratio. Mixtures of Val, Leu, Phe, Trp, Art and Ala derivatized with phenylisothiocyanate were separated using prepared stationary phases by HPLC. The results indicated that separation can be accomplished only by controlling column temperatures, which proves that chromatographic retention of analytes alters with the change of column temperature, and the materials are of temperature-responsive property.

AB - Chromatographic packing materials containing poly(N-isopropylacrylamide-co-2-acr-ylamido-2-methyl propane sulfonic acid) and poly(N-isopropylacrylamide-co-N-tert-butylacryla-mide) were prepared via conventional radical polymerization. Characterization was performed with thermogravimetric analysis (TGA) and elemental analysis to determine the grafting ratio. Mixtures of Val, Leu, Phe, Trp, Art and Ala derivatized with phenylisothiocyanate were separated using prepared stationary phases by HPLC. The results indicated that separation can be accomplished only by controlling column temperatures, which proves that chromatographic retention of analytes alters with the change of column temperature, and the materials are of temperature-responsive property.

KW - Chromatographic separation

KW - Poly(N-isopropylacrylamide)

KW - Temperature-responsive polymer

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SN - 1001-0645

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JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

IS - 9

ER -

Preparation of temperature-responsive chromatographic materials containing acidic groups for separation of amino acids

Abstract

Keywords

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