Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.026

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang^*, Ke Ming Shan, Yu Lin Deng

^*Corresponding author for this work

School of Medical and Technology

Research output: Contribution to journal › Article › peer-review

Abstract

An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

Original language	English
Pages (from-to)	119-122
Number of pages	4
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.026
Publication status	Published - 1 Jul 2015

Keywords

Biomarker
Diabetes
HPLC-ESI TOF MS
Human serum albumin
O-labeling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.15918/j.tbit1001-0645.2015.supplement1.026

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abstract = "An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.",

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AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

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AB - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

KW - Biomarker

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KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

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ER -

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Abstract

Keywords

UN SDGs

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