Abstract
In order to develop a biosensor based on porous structured cellulose microspheres for glucose detection with “signal-and-color” output, in this work, active group carboxyl was introduced to cellulose matrix by using plasma technology, and then glucose oxidase (GOx) was chemically immobilized through EDC-NHS cross-linking reaction. The cellulose microgels containing 21.28 mg/g of enzymes exhibited a fast response to 0.003 M glucose within only 4 min. As for detecting subject with a lower concentration of glucose, the probe still worked. When the concentration of glucose solution was 0.005 M, it took only 2 min that the reaction mixture changed from colorless to yellow. By the introduction of starch, the reaction mixture presented as amaranth color. Besides, the porous-structured substrate and the facile plasma technology were also promising for constructing enzyme-driven catalytic systems with enhanced performance.
Original language | English |
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Pages (from-to) | 295-301 |
Number of pages | 7 |
Journal | Carbohydrate Polymers |
Volume | 205 |
DOIs | |
Publication status | Published - 1 Feb 2019 |
Externally published | Yes |
Keywords
- Biosensor
- Cellulose
- Glucose detection
- Plasma grafting