Optimization of laser-induced fluorescence detection system for microfluidic chip

Pan Shi; Xiaoming Hu; Qin Li; Yulin Deng

doi:10.1109/ITAB.2008.4570634

Optimization of laser-induced fluorescence detection system for microfluidic chip

Pan Shi, Xiaoming Hu, Qin Li^*, Yulin Deng

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Laser-induced fluorescence (LIF) detection is widely utilized in microfluidic chip due to its high sensitivity and selectivity. In this paper, a LIF detection system for the microscope chip based on a 473 nm laser diode is described. To improve the performance of the detection system, the confocal configuration is adopted in our experiment. Some factors that are important to the signal-to-noise ratio (SNR) of the confocal system, such as the choice of microscope objective, effect of excitation source, the size of pinhole and the performance of the PMT detection units are presented and explained in this paper.

Original language	English
Title of host publication	5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Pages	207-210
Number of pages	4
DOIs	https://doi.org/10.1109/ITAB.2008.4570634
Publication status	Published - 2008
Event	5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 - Shenzhen, China Duration: 30 May 2008 → 31 May 2008

Publication series

Name	5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008

Conference

Conference	5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Country/Territory	China
City	Shenzhen
Period	30/05/08 → 31/05/08

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10.1109/ITAB.2008.4570634

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Shi, P., Hu, X., Li, Q., & Deng, Y. (2008). Optimization of laser-induced fluorescence detection system for microfluidic chip. In 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 (pp. 207-210). Article 4570634 (5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008). https://doi.org/10.1109/ITAB.2008.4570634

Shi, Pan ; Hu, Xiaoming ; Li, Qin et al. / Optimization of laser-induced fluorescence detection system for microfluidic chip. 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. pp. 207-210 (5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008).

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title = "Optimization of laser-induced fluorescence detection system for microfluidic chip",

abstract = "Laser-induced fluorescence (LIF) detection is widely utilized in microfluidic chip due to its high sensitivity and selectivity. In this paper, a LIF detection system for the microscope chip based on a 473 nm laser diode is described. To improve the performance of the detection system, the confocal configuration is adopted in our experiment. Some factors that are important to the signal-to-noise ratio (SNR) of the confocal system, such as the choice of microscope objective, effect of excitation source, the size of pinhole and the performance of the PMT detection units are presented and explained in this paper.",

author = "Pan Shi and Xiaoming Hu and Qin Li and Yulin Deng",

year = "2008",

doi = "10.1109/ITAB.2008.4570634",

language = "English",

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pages = "207--210",

booktitle = "5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008",

note = "5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 ; Conference date: 30-05-2008 Through 31-05-2008",

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Shi, P, Hu, X , Li, Q & Deng, Y 2008, Optimization of laser-induced fluorescence detection system for microfluidic chip. in 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008., 4570634, 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008, pp. 207-210, 5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008, Shenzhen, China, 30/05/08. https://doi.org/10.1109/ITAB.2008.4570634

Optimization of laser-induced fluorescence detection system for microfluidic chip. / Shi, Pan; Hu, Xiaoming ; Li, Qin et al.
5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. p. 207-210 4570634 (5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Laser-induced fluorescence (LIF) detection is widely utilized in microfluidic chip due to its high sensitivity and selectivity. In this paper, a LIF detection system for the microscope chip based on a 473 nm laser diode is described. To improve the performance of the detection system, the confocal configuration is adopted in our experiment. Some factors that are important to the signal-to-noise ratio (SNR) of the confocal system, such as the choice of microscope objective, effect of excitation source, the size of pinhole and the performance of the PMT detection units are presented and explained in this paper.

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Shi P, Hu X , Li Q , Deng Y. Optimization of laser-induced fluorescence detection system for microfluidic chip. In 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. p. 207-210. 4570634. (5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008). doi: 10.1109/ITAB.2008.4570634

Optimization of laser-induced fluorescence detection system for microfluidic chip

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