TY - JOUR
T1 - Optimization of dynamic immobilization of trypsin onto IAM
AU - Li, Hua
AU - Wang, Shanshan
AU - Lin, Zhenguang
AU - Qu, Feng
AU - Geng, Lina
AU - Li, Qin
AU - Deng, Yulin
PY - 2008
Y1 - 2008
N2 - In this study, Immobilized Artificial Membrane (IAM) was chosen as matrix and trypsin was used as immobilized macromolecule. The effect of different buffer solutions (Borate, Phosphate, and Tris HCl), concentrations, and pH on the immobilizing rate and activity of the immobilized trypsin was investigated. The optimal condition of off-line immobilization was Tris HCl buffer (10mmol/L, pH 8.0). On the basis of the obtained optimal condition, the trypsin enzyme reactor was prepared, and the preliminary result showed that the dynamically immobilized enzyme reactor possessed high activity, which can be specifically inhibited by the trypsin inhibitor.
AB - In this study, Immobilized Artificial Membrane (IAM) was chosen as matrix and trypsin was used as immobilized macromolecule. The effect of different buffer solutions (Borate, Phosphate, and Tris HCl), concentrations, and pH on the immobilizing rate and activity of the immobilized trypsin was investigated. The optimal condition of off-line immobilization was Tris HCl buffer (10mmol/L, pH 8.0). On the basis of the obtained optimal condition, the trypsin enzyme reactor was prepared, and the preliminary result showed that the dynamically immobilized enzyme reactor possessed high activity, which can be specifically inhibited by the trypsin inhibitor.
KW - Immobilization
KW - Immobilized artificial membrane (IAM)
KW - Trypsin
UR - http://www.scopus.com/inward/record.url?scp=50049085233&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:50049085233
SN - 0441-3776
VL - 71
SP - 473
EP - 476
JO - Chemistry Bulletin / Huaxue Tongbao
JF - Chemistry Bulletin / Huaxue Tongbao
IS - 6
ER -