Normobaric hypoxia-induced brain damage and mechanism in wistar rat

Dingyu Hu, Qin Li, Bo Li, Rongji Dai, Lina Geng, Yulin Deng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

To evaluate normobaric hypoxia-induced brain damage and discuss the mechanism in wistar rat brain injury, the water content of brain , brain infarction area ratio , the level of lactic acid and Na+-K +-ATPase activity were detected. The expression of HIF-1α was confirmed using immunohistochemistry. Histopathological changes of brain in rat model due to hypoxia were investigated using hematoxylin and eosin stain (HE). Results showed that the levels of lactic acid were positively correlated with the degree of hypoxia which caused a concentration-dependent decrease in total Na+-K+-ATPase activity. Compared with the control group, brain water content of rat model had significant difference under serious hypoxia condition. Brain infarction ratio increased sharply when lactic acid level increased and Na+-K+-ATPase activity decreased obviously. Histopathological examination confirmed that there was a necrotic feature in one single hippocampal gyrus neuron cell. HIF-1α (hypoxia induced factor-1α) was induced by hypoxia at 6% O2. Expression of HIF-1α enhanced the hypoxia adaptation capability of the rat model through regulating the expression of multiple genes. Lactic acid, Na +-K+-ATPase and HIF-1α played an important role in brain injury as a possible mechanism.

Original languageEnglish
Title of host publication3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
DOIs
Publication statusPublished - 2009
Event3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 - Beijing, China
Duration: 11 Jun 200913 Jun 2009

Publication series

Name3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Conference

Conference3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Country/TerritoryChina
CityBeijing
Period11/06/0913/06/09

Keywords

  • Brain damage
  • HIF-1α
  • Mechanism
  • Normobaric hypoxia

Fingerprint

Dive into the research topics of 'Normobaric hypoxia-induced brain damage and mechanism in wistar rat'. Together they form a unique fingerprint.

Cite this