TY - JOUR
T1 - Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography
AU - Zhang, Chunyu
AU - Zhang, Kai
AU - Cui, Yanyan
AU - Guo, Yuecong
AU - Wang, Chuan
AU - Xu, Chao
AU - Yao, Qingqiang
AU - Zhao, Yuliang
AU - Chen, Chunying
AU - Wang, Yaling
N1 - Publisher Copyright:
© 2022 American Chemical Society.
PY - 2023/1/31
Y1 - 2023/1/31
N2 - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.
AB - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.
UR - http://www.scopus.com/inward/record.url?scp=85145462578&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.2c03699
DO - 10.1021/acs.analchem.2c03699
M3 - Article
AN - SCOPUS:85145462578
SN - 0003-2700
VL - 95
SP - 2129
EP - 2133
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 4
ER -