Monitoring of early diagnosis of Alzheimer's disease using the cellular prion protein and poly(pyrrole-2-carboxylic acid) modified electrode

Jieling Qin; Dong Gyu Jo; Misuk Cho; Youngkwan Lee

doi:10.1016/j.bios.2018.04.061

Monitoring of early diagnosis of Alzheimer's disease using the cellular prion protein and poly(pyrrole-2-carboxylic acid) modified electrode

Jieling Qin, Dong Gyu Jo, Misuk Cho^*, Youngkwan Lee

^*Corresponding author for this work

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer’ disease (AD), and the cellular prion protein (PrP^C) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrP^C receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10⁻⁴ pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.

Original language	English
Pages (from-to)	82-87
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	113
DOIs	https://doi.org/10.1016/j.bios.2018.04.061
Publication status	Published - 15 Aug 2018
Externally published	Yes

Keywords

Alzheimer's disease
Amyloid beta oligomer
Cellular prion protein (PrP)
Impedance

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10.1016/j.bios.2018.04.061

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title = "Monitoring of early diagnosis of Alzheimer's disease using the cellular prion protein and poly(pyrrole-2-carboxylic acid) modified electrode",

abstract = "Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer{\textquoteright} disease (AD), and the cellular prion protein (PrPC) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrPC receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10−4 pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.",

keywords = "Alzheimer's disease, Amyloid beta oligomer, Cellular prion protein (PrP), Impedance",

author = "Jieling Qin and Jo, {Dong Gyu} and Misuk Cho and Youngkwan Lee",

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year = "2018",

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doi = "10.1016/j.bios.2018.04.061",

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AU - Qin, Jieling

AU - Jo, Dong Gyu

AU - Cho, Misuk

AU - Lee, Youngkwan

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer’ disease (AD), and the cellular prion protein (PrPC) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrPC receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10−4 pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.

AB - Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer’ disease (AD), and the cellular prion protein (PrPC) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrPC receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10−4 pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.

KW - Alzheimer's disease

KW - Amyloid beta oligomer

KW - Cellular prion protein (PrP)

KW - Impedance

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Monitoring of early diagnosis of Alzheimer's disease using the cellular prion protein and poly(pyrrole-2-carboxylic acid) modified electrode

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Keywords

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