TY - JOUR
T1 - Micromanipulation for Coiling Microfluidic Spun Alginate Microfibers by Magnetically Guided System
AU - Sun, Tao
AU - Wang, Huaping
AU - Shi, Qing
AU - Takeuchi, Masaru
AU - Nakajima, Masahiro
AU - Huang, Qiang
AU - Fukuda, Toshio
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/7
Y1 - 2016/7
N2 - Alginate hydrogel microfibers are a promising cell-laden module for three-dimensional (3-D) assembly to build cellular structures. However, it is still a challenge to manipulate them for microassembly. In this letter, we report a novel magnetic control method to handle this challenge. To enhance the controllability, we mix magnetic nanoparticles (MNPs) into alginate solution, and then spin magnetic alginate microfibers (MAMs) by a microfluidic device. To achieve precise magnetic control, we establish a magnetically guided system composed of a magnetic tweezer with a needle tip and a glass micropipette mounted on two manipulation robots, respectively. Moreover, a series of experiments have been designed to obtain the optimized solenoid current and tip structure of magnetic tweezer. Furthermore, a manipulation of coiling MAM around a micropillar at the microscale can be experimentally demonstrated. Based on such manipulation, a microassembly of MAM can be finally achieved to form a tube-shaped microstructure for 3-D cell culture.
AB - Alginate hydrogel microfibers are a promising cell-laden module for three-dimensional (3-D) assembly to build cellular structures. However, it is still a challenge to manipulate them for microassembly. In this letter, we report a novel magnetic control method to handle this challenge. To enhance the controllability, we mix magnetic nanoparticles (MNPs) into alginate solution, and then spin magnetic alginate microfibers (MAMs) by a microfluidic device. To achieve precise magnetic control, we establish a magnetically guided system composed of a magnetic tweezer with a needle tip and a glass micropipette mounted on two manipulation robots, respectively. Moreover, a series of experiments have been designed to obtain the optimized solenoid current and tip structure of magnetic tweezer. Furthermore, a manipulation of coiling MAM around a micropillar at the microscale can be experimentally demonstrated. Based on such manipulation, a microassembly of MAM can be finally achieved to form a tube-shaped microstructure for 3-D cell culture.
KW - Coiling process
KW - Micro-assembly
KW - alginate microfibers
KW - magnetic tweezerp
UR - http://www.scopus.com/inward/record.url?scp=85058585401&partnerID=8YFLogxK
U2 - 10.1109/LRA.2016.2524991
DO - 10.1109/LRA.2016.2524991
M3 - Article
AN - SCOPUS:85058585401
SN - 2377-3766
VL - 1
SP - 808
EP - 813
JO - IEEE Robotics and Automation Letters
JF - IEEE Robotics and Automation Letters
IS - 2
M1 - 7397894
ER -