TY - JOUR
T1 - Microfluidic chip with thermoresponsive boronate affinity for the capture-Release of cis-Diol biomolecules
AU - Man, Ya
AU - Peng, Guang
AU - Wang, Jianshe
AU - Lv, Xuefei
AU - Deng, Yulin
N1 - Publisher Copyright:
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - The polydimethylsiloxane microfluidic chip grafted with poly(N-isopropylacrylamide-3-acrylamidephenylboronic acid) (P(NIPAAm-co-AAPBA)) was fabricated by UV-induced grafting polymerization for the capture-release of cis-diol-containing biomolecules by temperature-modulated changes instead of changing the pH value of the mobile phase. Based on the optimal time for benzophenone soaking and UV irradiation of grafting polymerization, P(NIPAAm-co-AAPBA) was successfully grafted on the polydimethylsiloxane substrates, which were characterized by scanning electron microscopy, water contact angle measurements, and Fourier transform infrared spectroscopy. The P(NIPAAm-co-AAPBA)- grafted polydimethylsiloxane microfluidic chip can be successfully used for the capture and release of cis-diol-containing adenosine by adjusting the temperature from 4 to 55°C, and the result was validated by Triple Quad liquid chromatography with mass spectrometry. With further development, the fabricated polydimethylsiloxanemicrofluidic chipsmight be chosen as a potential tool for the capture and release of cis-diol-containing macromolecules, such as horseradish peroxidase and glycoprotein.
AB - The polydimethylsiloxane microfluidic chip grafted with poly(N-isopropylacrylamide-3-acrylamidephenylboronic acid) (P(NIPAAm-co-AAPBA)) was fabricated by UV-induced grafting polymerization for the capture-release of cis-diol-containing biomolecules by temperature-modulated changes instead of changing the pH value of the mobile phase. Based on the optimal time for benzophenone soaking and UV irradiation of grafting polymerization, P(NIPAAm-co-AAPBA) was successfully grafted on the polydimethylsiloxane substrates, which were characterized by scanning electron microscopy, water contact angle measurements, and Fourier transform infrared spectroscopy. The P(NIPAAm-co-AAPBA)- grafted polydimethylsiloxane microfluidic chip can be successfully used for the capture and release of cis-diol-containing adenosine by adjusting the temperature from 4 to 55°C, and the result was validated by Triple Quad liquid chromatography with mass spectrometry. With further development, the fabricated polydimethylsiloxanemicrofluidic chipsmight be chosen as a potential tool for the capture and release of cis-diol-containing macromolecules, such as horseradish peroxidase and glycoprotein.
KW - Boronate affinity
KW - Poly(N-isopropylacrylamide)
KW - Polydimethylsiloxane
KW - Thermoresponse
UR - http://www.scopus.com/inward/record.url?scp=84921550435&partnerID=8YFLogxK
U2 - 10.1002/jssc.201400725
DO - 10.1002/jssc.201400725
M3 - Article
C2 - 25376697
AN - SCOPUS:84921550435
SN - 1615-9306
VL - 38
SP - 339
EP - 345
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 2
ER -