TY - GEN
T1 - Mechanism study on mitochondrial fragmentation under oxidative stress caused by high fluence low-power laser irradiation
AU - Wu, Shengnan
AU - Zhou, Feifan
AU - Xing, Da
PY - 2012
Y1 - 2012
N2 - Mitochondria are dynamic organelles that undergo continual fusion and fission to maintain their morphology and functions, but the mechanism involved is still not clear. Here, we investigated the effect of mitochondrial oxidative stress triggered by high-fluence low-power laser irradiation (HF-LPLI) on mitochondrial dynamics in human lung adenocarcinoma cells (ASTC-a-1). Upon HF-LPLI-triggered oxidative stress, mitochondria displayed a fragmented structure, which was abolished by exposure to dehydroascorbic acid (DHA), a reactive oxygen species scavenger, indicating that oxidative stress can induce mitochondrial fragmentation. Mitochondrial translocation of the profission protein dynamin-related protein 1 (Drp1) was observed following HF-LPLI, demonstrating apoptosis-related activation of Drp1. Notably, DHA pre-treatment prevented HF-LPLI-induced Drp1 activation. We conclude that mitochondrial oxidative stress through activation of Drp1 causes mitochondrial fragmentation.
AB - Mitochondria are dynamic organelles that undergo continual fusion and fission to maintain their morphology and functions, but the mechanism involved is still not clear. Here, we investigated the effect of mitochondrial oxidative stress triggered by high-fluence low-power laser irradiation (HF-LPLI) on mitochondrial dynamics in human lung adenocarcinoma cells (ASTC-a-1). Upon HF-LPLI-triggered oxidative stress, mitochondria displayed a fragmented structure, which was abolished by exposure to dehydroascorbic acid (DHA), a reactive oxygen species scavenger, indicating that oxidative stress can induce mitochondrial fragmentation. Mitochondrial translocation of the profission protein dynamin-related protein 1 (Drp1) was observed following HF-LPLI, demonstrating apoptosis-related activation of Drp1. Notably, DHA pre-treatment prevented HF-LPLI-induced Drp1 activation. We conclude that mitochondrial oxidative stress through activation of Drp1 causes mitochondrial fragmentation.
KW - Dynamin-related protein 1 (Drp1)
KW - Fission
KW - High fluence low-power laser irradiation (HF-LPLI)
KW - Oxidative stress
KW - Reactive oxygen species (ROS)
UR - http://www.scopus.com/inward/record.url?scp=84859357926&partnerID=8YFLogxK
U2 - 10.1117/12.905332
DO - 10.1117/12.905332
M3 - Conference contribution
AN - SCOPUS:84859357926
SN - 9780819488541
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Mechanisms for Low-Light Therapy VII
T2 - Mechanisms for Low-Light Therapy VII
Y2 - 21 January 2012 through 21 January 2012
ER -