TY - GEN
T1 - Lectin chip for individally isolating of pancereatic circulating tumor cells
AU - Masuda, Taisuke
AU - Tateno, Hiroaki
AU - Lei, Wu
AU - Arifin, Izzuddin
AU - Arai, Fumihito
N1 - Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.
AB - To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.
KW - Cell Isolation
KW - Lection
KW - Microfluidic
KW - Pancreatic Cancer Cell
KW - Single-cell Recovery
UR - http://www.scopus.com/inward/record.url?scp=85079631815&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079631815
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 187
EP - 188
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -
