The composition of the In-Warhead data acquisition system, virtual instrument and its develop environment-LabVIEW, has been introduced in this paper. Then the possibility and facility of applying LabVIEW in In-Warhead data acquisition system has been discussed and a data acquisition program to read data from the acquisition board to PC and then display the data on the PC screen for further analysis has been made in a more convenient way. Practice shows that LabVIEW can shorten the developing time of systems of data acquisition, measure, etc.. Simultaneously, it increases the efficiency of programming.