TY - JOUR
T1 - Ionically Imprinting-Based Copper (II) Label-Free Detection for Preventing Hearing Loss
AU - Wang, Huan
AU - Zhang, Hui
AU - Zhang, Xiaoli
AU - Chen, Hong
AU - Lu, Ling
AU - Chai, Renjie
N1 - Publisher Copyright:
© 2023 Chinese Academy of Engineering
PY - 2024/2
Y1 - 2024/2
N2 - Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmol·L−1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.
AB - Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmol·L−1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.
KW - Hearing loss
KW - Ionic imprinting
KW - Label-free detection
KW - Microfluidics
KW - Structural color
UR - http://www.scopus.com/inward/record.url?scp=85172256318&partnerID=8YFLogxK
U2 - 10.1016/j.eng.2023.09.001
DO - 10.1016/j.eng.2023.09.001
M3 - Article
AN - SCOPUS:85172256318
SN - 2095-8099
VL - 33
SP - 276
EP - 282
JO - Engineering
JF - Engineering
ER -