Identifying Phase-Amplitude Coupling in Cyclic Alternating Pattern using Masking Signals

Judiciously classifying phase-A subtypes in cyclic alternating pattern (CAP) is critical for investigating sleep dynamics. Phase-amplitude coupling (PAC), one of the representative forms of neural rhythmic interaction, is defined as the amplitude of high-frequency activities modulated by the phase of low-frequency oscillations. To examine PACs under more or less synchronized conditions, we propose a nonlinear approach, named the masking phase-amplitude coupling (MPAC), to quantify physiological interactions between high (α/lowβ) and low (δ) frequency bands. The results reveal that the coupling intensity is generally the highest in subtype A1 and lowest in A3. MPACs among various physiological conditions/disorders (p < 0.0001) and sleep stages (p < 0.0001 except S4) are tested. MPACs are found significantly stronger in light sleep than deep sleep (p < 0.0001). Physiological conditions/disorders show similar order in MPACs. Phase-amplitude dependence between δ and α/lowβ oscillations are examined as well. δ phase tent to phase-locked to α/lowβ amplitude in subtype A1 more than the rest. These results suggest that an elevated δ-α/lowβ MPACs can reflect some synchronization in CAP. Therefore, MPAC can be a potential tool to investigate neural interactions between different time scales, and δ-α/lowβ MPAC can serve as a feasible biomarker for sleep microstructure.