Identification of early glycated peptides of albumin by a method of isotopic labeling combined with neutral loss scan

Rui Su; Yun Wang; Ya Hui Liu; Jie Hong; Yong Qian Zhang; Yu Juan Li; Hong Qing; Yu Lin Deng

Identification of early glycated peptides of albumin by a method of isotopic labeling combined with neutral loss scan

Rui Su, Yun Wang, Ya Hui Liu, Jie Hong, Yong Qian Zhang, Yu Juan Li, Hong Qing, Yu Lin Deng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This project utilized isotopic labeling with [¹³C6] glucose as a tag, establishing non-enzymatic glycation reaction model in vitro. Glycated the model human serum albumin (HSA) was verified using MALDI-TOF. The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass (HPLC/Q-TOF MS) analysis platform. The 34 screened glycated peptides were analyzed using target MS/MS mode. The pFind software, which is a proteomics data analysis tool, was applied for the automated data analysis of the neutral scan and LC/MS/MS data. Finally, this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides.

Original language	English
Pages (from-to)	1310-1314
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	34
Issue number	12
Publication status	Published - 1 Dec 2014

Keywords

Glycated peptides
Isotopic label
Neutral loss

Cite this

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title = "Identification of early glycated peptides of albumin by a method of isotopic labeling combined with neutral loss scan",

abstract = "This project utilized isotopic labeling with [13C6] glucose as a tag, establishing non-enzymatic glycation reaction model in vitro. Glycated the model human serum albumin (HSA) was verified using MALDI-TOF. The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass (HPLC/Q-TOF MS) analysis platform. The 34 screened glycated peptides were analyzed using target MS/MS mode. The pFind software, which is a proteomics data analysis tool, was applied for the automated data analysis of the neutral scan and LC/MS/MS data. Finally, this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides.",

keywords = "Glycated peptides, Isotopic label, Neutral loss",

author = "Rui Su and Yun Wang and Liu, {Ya Hui} and Jie Hong and Zhang, {Yong Qian} and Li, {Yu Juan} and Hong Qing and Deng, {Yu Lin}",

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T1 - Identification of early glycated peptides of albumin by a method of isotopic labeling combined with neutral loss scan

AU - Su, Rui

AU - Wang, Yun

AU - Liu, Ya Hui

AU - Hong, Jie

AU - Zhang, Yong Qian

AU - Li, Yu Juan

AU - Qing, Hong

AU - Deng, Yu Lin

PY - 2014/12/1

Y1 - 2014/12/1

N2 - This project utilized isotopic labeling with [13C6] glucose as a tag, establishing non-enzymatic glycation reaction model in vitro. Glycated the model human serum albumin (HSA) was verified using MALDI-TOF. The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass (HPLC/Q-TOF MS) analysis platform. The 34 screened glycated peptides were analyzed using target MS/MS mode. The pFind software, which is a proteomics data analysis tool, was applied for the automated data analysis of the neutral scan and LC/MS/MS data. Finally, this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides.

AB - This project utilized isotopic labeling with [13C6] glucose as a tag, establishing non-enzymatic glycation reaction model in vitro. Glycated the model human serum albumin (HSA) was verified using MALDI-TOF. The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass (HPLC/Q-TOF MS) analysis platform. The 34 screened glycated peptides were analyzed using target MS/MS mode. The pFind software, which is a proteomics data analysis tool, was applied for the automated data analysis of the neutral scan and LC/MS/MS data. Finally, this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides.

KW - Glycated peptides

KW - Isotopic label

KW - Neutral loss

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M3 - Article

AN - SCOPUS:84922380382

SN - 1001-0645

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

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ER -

Identification of early glycated peptides of albumin by a method of isotopic labeling combined with neutral loss scan

Abstract

Keywords

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