High-Density Microneedle Array (HIDMA): An in vivo Electroporation Method for Low-Voltage Gene Delivery

Junshi Li, Tangren Yang, Dong Huang, Yufeng Chen, Yuanyu Huang, Zhihong Li*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

In this work, we report a novel in vivo electroporation method for gene delivery utilizing high-density microneedle array (HIDMA). Owing to the minimized needle spacing, hexagonal distribution, and tri-polarity electric connection of the device, the low voltage and uniform electric field during electroporation can dramatically reduce cell damage and enhance delivery efficiency. The HIDMA is constructed with modified acupuncture needles, and can be customized for both superficial and deep tissues with different electroporation areas. According to the in vivo experiments on DNA plasmid delivery, our 0.6 and 1.2-mm-long microneedles with ∼ 5μm tips and 750μm pitch, can penetrate the outer skin to reduce the contact impedance that achieves effective electroporation within tens of volts and without obvious injury.

Original languageEnglish
Title of host publication33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages372-375
Number of pages4
ISBN (Electronic)9781728135809
DOIs
Publication statusPublished - Jan 2020
Event33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada
Duration: 18 Jan 202022 Jan 2020

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2020-January
ISSN (Print)1084-6999

Conference

Conference33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Country/TerritoryCanada
CityVancouver
Period18/01/2022/01/20

Keywords

  • gene delivery
  • in vivo electroporation
  • low voltage
  • microneedle array

Fingerprint

Dive into the research topics of 'High-Density Microneedle Array (HIDMA): An in vivo Electroporation Method for Low-Voltage Gene Delivery'. Together they form a unique fingerprint.

Cite this