Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

Jieling Qin; Soochan Kim; Misuk Cho; Youngkwan Lee

doi:10.1016/j.cej.2020.126055

Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

Jieling Qin, Soochan Kim, Misuk Cho^*, Youngkwan Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrP^C) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrP^C biosensor showed a proportional relationship to the increase of AβO concentration from 10⁻⁹ to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

Original language	English
Article number	126055
Journal	Chemical Engineering Journal
Volume	401
DOIs	https://doi.org/10.1016/j.cej.2020.126055
Publication status	Published - 1 Dec 2020
Externally published	Yes

Keywords

Amyloid beta oligomer
Functional conductive polymer
Hierarchical and dendritic structure
Impedance sensor

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10.1016/j.cej.2020.126055

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title = "Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications",

abstract = "In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.",

keywords = "Amyloid beta oligomer, Functional conductive polymer, Hierarchical and dendritic structure, Impedance sensor",

author = "Jieling Qin and Soochan Kim and Misuk Cho and Youngkwan Lee",

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doi = "10.1016/j.cej.2020.126055",

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T1 - Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

AU - Qin, Jieling

AU - Kim, Soochan

AU - Cho, Misuk

AU - Lee, Youngkwan

PY - 2020/12/1

Y1 - 2020/12/1

N2 - In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

AB - In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

KW - Amyloid beta oligomer

KW - Functional conductive polymer

KW - Hierarchical and dendritic structure

KW - Impedance sensor

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VL - 401

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Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

Abstract

Keywords

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